The invention relates to a method of determining intrinsic parameters of a parabolic catadioptric camera through linearity of two mutually-shielded spheres. The method comprises the following steps that five images of a target are shot from different directions by utilizing the parabolic catadioptric camera, parabolic catadioptric images of the target are two intersected quadratic curves, boundary pixel point coordinates and image point coordinates of the five target images are extracted from the images, antipodal image points are obtained according to the relation between the image points and the antipodal image points, curve equations are respectively fitted, two groups of antipodal image points are formed by two actual points of intersection of a sphere image and two actual points of intersection of an antipodal sphere image, through definition of the antipodal image points, the two groups of antipodal image points provide one group of orthogonal direction end points on an image plane, and the intrinsic parameters of the camera are solved by utilizing constraint linearity of the orthogonal direction end points on an absolute conic image. By utilizing the target in the invention, full-automatic calibration can be implemented, and errors caused by measurement in a calibration process are reduced. Projection contour lines of the spheres can be completely extracted in the image, so that the calibration precision of the camera is improved.