Method for mass production of seedling of Jatropha curcas L.

Abstract

A method of mass production grease tung seed sprout through tissue culture, comprising the following steps: 1. inoculating the stem tip into the culture medium added with corn starch with 20-40% by weight: MS+6-BA 0.5-1.5mg / L+NAA 0.2-2mg / L+sugar 20-40g / L, solidifying with agar of 3-5%, culturing with light, with pH being 5.8 to 6, temperature in the culture room being 26+ / -2 Deg C, light intensity being 1000-2000lux, lighting hour being 10-14 hours every day, culturing for 20-28 days to form callus. 2. Cutting the callus and transferring it to the same culture medium for successive culture, and getting the tissue culture bud. 3. inoculating the tissue culture bud of bred multi-period and in mass to the following root-growing culture medium to get the tissue culture sprout: IBA0.3-0.6mg / L+MS, the root growing out 10-20 days later. 4. Replanting the tissue culture sprout out of bottle. The invention is characterized by the solution of problem of fast breeding grease tung using tissue culture, short period, low cost, and capability of meeting demand of large-area cultivating grease tung, it provides a high effective way for the grease tungartificial breeding, germ-free sprout producing and species of high quality breeding.

Description

technical field [0001] The invention belongs to the technical field of production of plant seedlings, and in particular relates to a method for large-scale production of tung tree seedlings through tissue culture. Background technique [0002] Jatropha curcas L. is also known as Jatropha curcas and Jatropha curcas. Distributed in tropical and subtropical regions. my country has cultivated or semi-wild. Shrubs or small trees, 2-5m high, much branched. fast-growing. Like fertile soil, acid soil or calcareous soil are suitable for growth. Resistant to low temperature around 0℃ and light frost. Strong wind resistance. my country's South China Sea islands are distributed. Cultivated or semi-wild in Taiwan, Fujian, Guangdong, Qiong, Guangxi, Yunnan, Guizhou, Sichuan and other places. It is mostly cultivated for fences and for ornamental purposes. The seed kernel contains about 50% oil, which can be used as lubricating oil, soap making, etc., and can induce vomiting and di...

AI Technical Summary

Problems solved by technology

Because its conditions can be strictly controlled and it grows rapidly, a period of about 1 month is a cycle, so tissue culture is called rapid propagation. If Tung tree can be used for rapid propagation in tissue culture, it will have an important application in the production of Tung tree seedlings value, but so far there is no report and use of tissue culture for rapid propagation of A. japonicus