Long chain diprotic acid refining method

Abstract

The invention relates to a method to extract and refine long-chain diacid from normal alkane fermentation liquor in water-phase, which comprises: adding proper urea when heating and breaking emulsion to overcome heavy emulsification and increase recycle yield of un-reacted alkane, decolourizing with adsorbent before degerming to save filter step and reduce cost.

Description

technical field

[0001] The invention relates to a method for refining long-chain dibasic acids from normal alkane fermentation broth, especially from C 10 ~C 18 A method for refining long-chain dibasic acids in n-alkane fermentation broth. Background technique

[0002] Long-chain dibasic acids are metabolites obtained from microbial fermentation of long-chain n-alkanes. Its fermentation broth is a complex multiphase system, which contains water, unreacted alkanes and various medium components, microbial cells, autolyzed cell fragments and proteins, pigments, various metabolites, long-chain dibasic acids , as well as emulsions of oil, water, and cells. This complex heterogeneous system increases the difficulty of extracting dibasic acids and affects the purity and yield of products.

[0003] At present, the methods for extracting long-chain dibasic acids are generally divided into solvent method and aqueous method. Although the solvent method can solve the above problems...