Method for detecting citrinin content in red koji fermentation product
A technology for fermented products and detection methods, applied in separation methods, chemical instruments and methods, measuring devices, etc., can solve problems such as difficulties in the export of fermented red yeast rice products, achieve reasonable extraction and concentration steps, short concentration time, and improve sensitivity Effect
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Embodiment 1
[0059] Determination of citrinin content in embodiment 1 XX red yeast rice
[0060] 1 Experimental materials
[0061] 1.1 Sample: Functional red yeast rice produced by XX Bioengineering Co., Ltd., which is red powder. About 100g / bag, 1 bag in total. Store in a cool place away from light.
[0062] 1.2 Main instruments: electronic balance, high performance liquid chromatography, spectrophotometer, acidity meter, ultrasonic cleaner, rotary evaporator, ultrapure water preparation system, vacuum pump, constant temperature water bath, 1×20cm chromatography column
[0063]1.3 Reference standard substance: citrinin (standard product, batch number: 1150490 34704019, Fluka, USA)
[0064] 1.4 Reagents: phosphoric acid (analytical grade, Beijing Chemical Plant, batch number: 20040408); acetonitrile (chromatographically pure, Fisher, USA, batch number: 052064); methanol (analytical grade, Beijing Chemical Factory, batch number: 20051022); macroporous adsorption resin (HPD -300, Cangzho...
Embodiment 3
[0093] Determination of Citrinin Content in Embodiment 3 XX Monascus Pigment
[0094] 1 Experimental materials
[0095] 1.1 Sample: xx Monascus pigment provided by XX Research Institute, which is red powder. About 50g / bag, 1 bag in total. Store in a cool place away from light.
[0096] 1.2 Main instruments: electronic balance, high performance liquid chromatography, spectrophotometer, acidity meter, ultrasonic cleaner, rotary evaporator, ultrapure water preparation system, vacuum pump, constant temperature water bath, 1×20cm chromatography column
[0097] 1.3 Reference standard substance: citrinin (standard product, batch number: 1150490 34704019, Fluka, USA)
[0098] 1.4 Reagents: phosphoric acid (analytical grade, Beijing Chemical Plant, batch number: 20040408); acetonitrile (chromatographically pure, Fisher, USA, batch number: 052064); methanol (analytical grade, Beijing Chemical Factory, batch number: 20051022); macroporous adsorption resin (HPD -300, Cangzhou Baoen Ch...
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