Immobilized microorganism for biomass fermentation and hydrogen production and preparation method thereof
A technology of immobilized microorganisms and biomass fermentation, applied in biochemical equipment and methods, immobilized enzymes, fermentation, etc., to achieve the effects of stable fermentation hydrogen production, large gas production, and increasing income of poor farmers
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Embodiment 1
[0042] Example 1, Glucose as the raw material mixed strain immobilized microbial fermentation hydrogen production
[0043] Using glucose as the raw material and the substrate at three concentrations of 1%, 7.5% and 15% respectively, use a 1L bioreactor device to ferment and produce hydrogen, with a liquid volume of 800mL, using the mixed strain immobilized microorganism technology of the present invention 100 g of immobilized blocks made by the method were fermented in batches at 30°C to produce argon. The results are shown in Table 1 below,
[0044] Table 1 Hydrogen production by immobilized fermentation at different concentrations of glucose
[0045]
[0046] Note: Vmax refers to the maximum hydrogen production rate: Vaverage refers to the average rate of hydrogen production in the entire fermentation process: YH 2 Refers to the yield of hydrogen gas
[0047] At the concentration of 1%, 7.5% and 15% glucose, mixed strain immobilized microorganism fermented to produce h...
Embodiment 2
[0048] Example 2, sucrose as raw material mixed strain immobilized microbial fermentation hydrogen production
[0049] The sucrose was formulated to a concentration of 15%, and the fermentative hydrogen production was started according to the method in Example 1. The maximum hydrogen content is 51.46%, and the average content is 45.56%; the maximum hydrogen production rate is 324.20mL / L.h, and the average hydrogen production rate is 127.91mL / L.h; the hydrogen production rate is 3.20mol / mol sucrose. The present invention uses sucrose as raw material to produce hydrogen by fermentation and compares the different technologies reported in the literature in Table 2. It can be seen that compared with non-immobilized or related immobilized technologies, the hydrogen produced by sucrose fermentation of the present invention has the largest hydrogen production The rate is the fastest, and the hydrogen production is also the highest. Only the mixed microorganisms carried by activated ca...
Embodiment 3
[0052] Example 3, lactose as raw material mixed strain immobilized microbial fermentation hydrogen production
[0053] The lactose was formulated to a concentration of 15%, and the fermentative hydrogen production test was started according to the method in Example 1. The results showed that the highest hydrogen content was 46.73%, and the average content was 36.89%; the maximum hydrogen production rate was 138.46mL / L.h, and the average hydrogen production rate was 69.24mL / L.h; the hydrogen production rate was 1.72mol / mol lactose. In 2004, Christophe Collet et al. used Clostridium thermolacticum (a strain that can efficiently utilize lactose fermentation) to continuously ferment hydrogen at 58°C, and obtained a maximum hydrogen production rate of 63.0ml / L.h and a hydrogen production of 1.5mol / mol lactose. The maximum hydrogen production rate and hydrogen gas rate obtained in this experiment are respectively increased by 119.8% and 14.7%.
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