Unlock instant, AI-driven research and patent intelligence for your innovation.

Immobilized microorganism for biomass fermentation and hydrogen production and preparation method thereof

A technology for immobilizing microorganisms and biomass fermentation, applied in biochemical equipment and methods, immobilizing enzymes, fermentation, etc., to achieve the effects of stabilizing the source of microbial provenance, high gas production, and high efficiency

Inactive Publication Date: 2006-06-21
YUNNAN NORMAL UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are a series of problems to be solved for the formulation, production and operation of immobilization technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immobilized microorganism for biomass fermentation and hydrogen production and preparation method thereof
  • Immobilized microorganism for biomass fermentation and hydrogen production and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, Glucose as the raw material mixed strain immobilized microbial fermentation hydrogen production

[0043] Using glucose as the raw material and the substrate at three concentrations of 1%, 7.5% and 15% respectively, use a 1L bioreactor device to ferment and produce hydrogen, with a liquid volume of 800mL, using the mixed strain immobilized microorganism technology of the present invention 100 g of immobilized blocks made by the method were fermented in batches to produce hydrogen at 30°C. The results are shown in Table 1 below,

[0044] glucose

h 2 Maximum content%

Average H 2 Content%

Vmax(mL / L.h)

V average (mL / L.h)

YH2(mol / mol)

1％

7.5％

15％

43.61

50.84

47.91

40.12

43.73

45.45

130.83

335.54

373.70

46.06

130.34

182.38

1.11

1.34

1.30

[0045] Note: Vmax refers to the maximum hydrogen production rate: Vaverage refers to the average rate of hydrogen ...

Embodiment 2

[0047] Example 2, sucrose as raw material mixed strain immobilized microbial fermentation hydrogen production

[0048] The sucrose was formulated to a concentration of 15%, and the fermentative hydrogen production was started according to the method in Example 1. The maximum hydrogen content is 51.46%, and the average content is 45.56%; the maximum hydrogen production rate is 324.20mL / L.h, and the average hydrogen production rate is 127.91mL / L.h; the hydrogen production rate is 3.20mol / mol sucrose. The present invention uses sucrose as raw material to produce hydrogen by fermentation and compares the different technologies reported in the literature in Table 2. It can be seen that compared with non-immobilized or related immobilized technologies, the hydrogen produced by sucrose fermentation of the present invention has the largest hydrogen production The rate is the fastest, and the hydrogen production is also the highest. Only the mixed microorganisms carried by activated ca...

Embodiment 3

[0050] Example 3, lactose as raw material mixed strain immobilized microbial fermentation hydrogen production

[0051] The lactose was formulated to a concentration of 15%, and the fermentative hydrogen production test was started according to the method in Example 1. The results showed that the highest hydrogen content was 46.73%, and the average content was 36.89%; the maximum hydrogen production rate was 138.46mL / L.h, and the average hydrogen production rate was 69.24mL / L.h; the hydrogen production rate was 1.72mol / mol lactose. In 2004, Christophe Collet et al. used Clostridium thermolacticum (a strain that can efficiently utilize lactose fermentation) to continuously ferment hydrogen at 58°C, and obtained a maximum hydrogen production rate of 63.0ml / L.h and a hydrogen production of 1.5mol / mol lactose. The maximum hydrogen production rate and hydrogen gas rate obtained in this experiment are respectively increased by 119.8% and 14.7%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates biomass fermented and immobilized microorganism and enzyme and preparing method. The method comprises the following components: polyvinyl alcohol glue solution, bacterial flora liquid (dehydrogenase enzymatic activity í¦50U / mL) comprising the single bacterial flora liquid and miscegenation bacterial flora liquid, 8-16% alta-mud (adsorbing carrier), and auxiliary agent: 10-20% CaCO3, 0.1-0.2% sulfo-aminolactic acid or cysteine, sulphate cation M(M=Fe2+,Ni2+,Fe3+, Mg2+,K+,Mn2+), ammonium salt anion N(N= [Mo7O24]6-, [C2O4]2-, [C6H5O7]3- ,SO42-), and 1% Na2S. The method uses the sugar S (S= xylo-pfan, lactose, grape-sugar, cane sugar, amylogen, potato) and fresh potato hydrolysate as raw material to continuously ferment and produce hydrogen. The invention provides the continuous, highly effective and stable microbial source, and the method is used for different biomass raw materials to ferment and produce hydrogen.

Description

Technical field [0001] The invention relates to immobilized microorganisms and enzymes for hydrogen production by biomass fermentation and a preparation method thereof. technical background [0002] Hydrogen energy is clean, efficient and renewable energy. Biomass has a wide range of raw materials, and the use of biomass to prepare hydrogen energy can deal with organic pollutants, which is an important technology in the field of renewable alternative energy research. [0003] In 1937, Nakamura first reported the phenomenon of biological hydrogen production. In the early 1980s, Japanese scholar Suzuki et al. used cells to immobilize Clostridium to produce hydrogen using alcohol factory wastewater as a substrate. In the 1990s, anaerobic activated sludge was directly used as natural hydrogen-producing microorganisms, and carbohydrates were used as hydrogen donors to produce biohydrogen through anaerobic fermentation, which made substantial progres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/00C12N11/02C12N11/04C12N11/08C12P3/00
Inventor 刘士清张无敌马欢
Owner YUNNAN NORMAL UNIV