Preparation process of nanometer carboxymethyl chitosan particle as medicine carrier
A technology of carboxymethyl chitosan and nanoparticles, which can be used in pharmaceutical formulations, medical preparations of non-active ingredients, powder delivery, etc. It can solve the problems of large energy requirements and side effects of organic solvents, and meet environmental protection requirements , avoid side effects, process safety effect
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Embodiment 1
[0013] A kind of preparation method of drug carrier carboxymethyl chitosan nano particle, it comprises the steps:
[0014] 1). Dissolve carboxymethyl chitosan in distilled water at room temperature to obtain a carboxymethyl chitosan aqueous solution with a concentration of 0.5 mg / mL; 2). Add 0.1M carboxymethyl chitosan dropwise to the carboxymethyl chitosan aqueous solution The hydrochloric acid adjustment carboxymethyl chitosan aqueous solution pH value is 1, under stirring condition, adding concentration is 0.3mg / mL sodium tripolyphosphate aqueous solution, press carboxymethyl chitosan aqueous solution: sodium tripolyphosphate volume aqueous solution ratio= 1:1.5, adding sodium tripolyphosphate aqueous solution to obtain a mixture; 3). The obtained mixture was stirred until the cross-linking was complete, then centrifuged at a high speed, and freeze-dried to obtain drug carrier carboxymethyl chitosan nanoparticles with a particle size of 90-220nm .
[0015] When encapsulati...
Embodiment 2
[0017] A kind of preparation method of drug carrier carboxymethyl chitosan nano particle, it comprises the steps:
[0018] 1). Dissolve carboxymethyl chitosan in distilled water at room temperature to obtain a carboxymethyl chitosan aqueous solution with a concentration of 2.0mg / mL; 2). Add 0.1M carboxymethyl chitosan dropwise to the carboxymethyl chitosan aqueous solution sodium hydroxide to adjust the pH value of carboxymethyl chitosan aqueous solution to 6, and under stirring conditions, adding a concentration of 1.8 mg / mL calcium chloride aqueous solution, according to the volume ratio of carboxymethyl chitosan aqueous solution: calcium chloride aqueous solution =1:2.5, add calcium chloride aqueous solution to obtain the mixture; 3). The obtained mixture is stirred until the cross-linking is complete, high-speed centrifugation, freeze-drying to obtain drug carrier carboxymethyl chitosan nanoparticles with a particle size of 90-220nm .
[0019] When encapsulating the medic...
Embodiment 3
[0021] A preparation method of drug-loaded carboxymethyl chitosan nanoparticles, at room temperature, 40mg carboxymethyl chitosan is dissolved in 20mL distilled water, and the obtained concentration is 2.0mg / mL carboxymethyl chitosan aqueous solution; add 0.1M hydrochloric acid dropwise to the carboxymethyl chitosan aqueous solution to adjust the pH value of the carboxymethyl chitosan aqueous solution to be 2, then dissolve 10 mg of the model drug bovine serum albumin in the carboxymethyl chitosan solution, Serum albumin concentration was 0.5 mg / mL; then 4 mL of 1.4 mg / mL sodium tripolyphosphate aqueous solution was added to 2 mL of carboxymethyl chitosan solution to obtain a mixture; the mixture was stirred at room temperature until cross-linking was complete, then centrifuged at 12000rmp Separating and freeze-drying to obtain drug-loaded carboxymethyl chitosan nanoparticles with a particle diameter of 90-220 nm. The prepared nanoparticles are more regular spherical. The enc...
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