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Extract of hippophae rhamnoides seed

A technology of extracts and seeds, applied in the field of seabuckthorn seed extracts, can solve problems such as multiple side effects, and achieve the effect of reducing eosinophils

Inactive Publication Date: 2008-07-16
NIHON UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compound drugs commonly used now also have more side effects

Method used

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  • Extract of hippophae rhamnoides seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of Seabuckthorn Seed Extract

[0025] 6.7kg seabuckthorn seeds were pretreated by screening and removing impurities, and soaked for 12 hours with 55L of 80% ethanol with a volume ratio of ethanol:water=8:2 (the same below). Then extract again with 55L of 80% ethanol, combine these extracts, concentrate under reduced pressure, and vacuum dry to obtain 1384.9g brown powder extract.

[0026] 674.2 g of the powder extract was dissolved in 2 L of water, and extracted three times with 2 L of diethyl ether, 2 L of ethyl acetate, and 2 L of butanol as extraction solutions. The ether fraction was then concentrated to yield 436.5 g of a brown oil. Among the above-mentioned components, take 7.42g of the ether component and concentrate the extract to put it on a silica gel column (Wako Silica Gel C-300 Wako Pure Chemical Industries, 5.5×15cm), and use (1) hexane 1L, (2) 5% ethyl acetate 2.5L, (3) 10% ethyl acetate 2L, (4) 15% ethyl acetate 2L, (5) 20% ethyl...

Embodiment 2

[0027] Example 2 Eosinophilic Apoptosis Induction Assay and MBP Kinase Activity Assay

[0028] Eosinophilic apoptosis induction test: The test was carried out according to the method of Vermes et al. (Journal of Immunological Methods 184, pp. 39-51, 1995). Make eosinophilic HL-60 cells float in RPMI1640 medium, the cell concentration is 1-3×10 6 cells / ml. Add the substance to be tested (one of the last collected components in Example 1 above) or the culture medium to 500 μl of the cell suspension to a concentration of 100 μg / ml, and store at 37° C., 5% CO 2 Incubate for 6 hours or 24 hours under the same conditions. After washing by centrifugation, phosphatidyl binding protein (Annexin) V buffer was added, and then 5 μl of phosphatidyl binding protein V-FITC was added. By flow cytometry, phosphatidyl binding protein V positive cells were regarded as apoptosis-inducing cells, and evaluated by the percentage relative to the total cell number.

[0029] MBP kinase activity t...

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Abstract

The present invention discloses extract of Hippophae rhamnoides seed having effects of inducing apoptosis, antihypersensitivity and antiinflammation. The present extract can be obtained by a method comprising extracting Hippophae rhamnoides seed with ethanol twice, concentrating the extract combined under reduced pressure, dissolving the resulting brown residue in water, extracting sequentially with ethyl ether, ethyl acetate, butanol three times respectively, concentrating ether phase, applying the resulting brown oil to silica gel column, eluting with ethyl acetate, and collecting the elutes. The present extract has the activity of inducing apoptosis of eosinophil granulocyte; can activate MBP kinase; and serve to induce apoptosis of eosinophil granulocyte in various diseases caused by excess eosinophil granulocyte.

Description

technical field [0001] The invention relates to a seabuckthorn seed extract which has the functions of inducing apoptosis, anti-allergic and anti-inflammation. Background technique [0002] Seabuckthorn (Hippophae rhamnoides L.) is a medicinal plant of the family Echinaceae that has been used in China for a long time. It is wild in the temperate arid regions of Tibet and Europe. Because seabuckthorn has formed a series of stress-resistant vitamins and biologically active natural substances during the long evolution process in nature, a variety of substances with strong physiological activities can be extracted from seabuckthorn seeds, which can effectively regulate blood lipids and prevent And treatment of coronary heart disease, angina pectoris and other cardiovascular and cerebrovascular diseases, anti-radiation, anti-inflammation, etc. The classic work of Tibetan medicine in my country, "Four Medical Codes", collects and records 7 kinds of mixtures made of seabuckthorn, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P11/02A61P11/08A61K131/00
Inventor 王珏北中进草间贞木泽靖夫古谷美野里
Owner NIHON UNIVERSITY
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