Alpha-acetyl lactate dehydrogenase dry powder and its preparation process

A technology of acetolactate decarboxylase and dry powder, which is applied in the directions of biochemical equipment and methods, food preparation, enzymes, etc., can solve the problems of difficult storage and transportation, poor thermal stability, etc.

Inactive Publication Date: 2009-01-07
南宁邦尔克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the α-acetolactate decarboxylase produced by manufacturers at home and abroad are all in liquid dosage form, which has poor thermal stability and is not easy to store and transport.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Prepare the protective agent enzyme solution according to the following components: (w / v)

[0013] Concentrated enzyme solution 100ml

[0014] 10 grams of protective agent, of which:

[0015] 5 parts trehalose

[0016] 0.08 parts of xanthan gum

[0017] 25 parts of maltodextrin

[0018] 0.42 parts of sodium alginate

[0019] 2. Add the components of the protective agent to the enzyme solution, stir until completely dissolved, and then set aside.

[0020] 3. Perform secondary homogenization on a high-pressure homogenizer with a working pressure of 30-40 MPa and a working flow of 1 liter / min.

[0021] 4. Spray and dry on a high-speed centrifugal spray dryer with an inlet air temperature of 150°C, an exhaust air temperature of 90°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 30 ml / min. Spray dry enzyme powder.

[0022] 5. Detect its enzyme activity and water content, the enzyme activity should be ≥10000u / g, and the water content sh...

Embodiment 2

[0025] 1. Prepare the protective agent enzyme solution according to the following components: (w / v)

[0026] Concentrated enzyme solution 100ml

[0027] 1 gram of protective agent, of which:

[0028] 20 parts trehalose

[0029] 0.12 parts of xanthan gum

[0030] 10 parts of β-cyclodextrin

[0031] 2.0 parts of sodium alginate

[0032] 2. Add the components of the protective agent to the enzyme solution, stir until completely dissolved, and then set aside.

[0033] 3. Perform a homogenization on a high-pressure homogenizer with a working pressure of 30-40MPa and a working flow of 1 liter / min and then standby.

[0034] 4. Spray drying on a high-speed centrifugal spray dryer with an inlet air temperature of 160°C, an exhaust air temperature of 80°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 40 ml / min. Spray dry enzyme powder.

[0035] 5. Detect its enzyme activity and water content, the enzyme activity should be ≥10000u / g, and the water con...

Embodiment 3

[0038] 1. Prepare the protective agent enzyme solution according to the following components: (w / v)

[0039] Concentrated enzyme solution 100ml

[0040] 30 grams of protective agent, of which:

[0041] 15 parts trehalose

[0042] 0.15 parts of xanthan gum

[0043] 15 parts of maltodextrin

[0044] 0.35 parts of sodium alginate

[0045] 2. Add the components of the protective agent to the enzyme solution, stir until completely dissolved, and then set aside.

[0046] 3. Perform secondary homogenization on a high-pressure homogenizer with a working pressure of 30-40 MPa and a working flow of 1 liter / min.

[0047] 4. Spray drying on a high-speed centrifugal spray dryer with an inlet air temperature of 170°C, an exhaust air temperature of 80°C, a cyclone separation tower pressure difference of 0.7×100KPa, and a feed flow rate of 50 ml / min. Spray dry enzyme powder.

[0048] 5. Test its enzyme activity and water content, the enzyme activity should be ≥10000u / g, and the water content shou...

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PUM

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Abstract

An alpha-acetolactate decarboxylase powder is prepared from the alpha-acetolactate decarboxylase liquid and the spray-frying protector composed of mycose, xanthan gum, sodium alginate and dextrin through spray drying. Its advantage is high stability.

Description

Technical field [0001] The invention relates to a food-added biological product of an enzyme preparation, in particular to a dry powder of α-acetolactate decarboxylase and a preparation method thereof. Background technique [0002] Alpha-acetolactate decarboxylase (ALDC, EC4.1.1.5) has been widely used by beer manufacturers as an auxiliary for beer maturation. In the beer brewing process, α-acetolactate undergoes non-enzymatic oxidative decarboxylation to form diacetyl. When the content of diacetyl exceeds 0.15mg / kg, it will seriously affect the taste of beer. The traditional method of removing diacetyl is to store beer at a low temperature, and let the diacetyl reductase produced by the yeast itself finally reduce the diacetyl to 2,3 butanediol. This process is called beer maturation, which usually takes 3 to 6 weeks. Up to 3 months. When this enzyme is applied to beer production, it can directly oxidatively decarboxylate α-acetolactate into acetoin, without the intermediate pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C12N9/88A23L1/305
Inventor 赵文报蒙健宗黄日波王青艳韦函忠周志鹏蒙丽萍马少敏
Owner 南宁邦尔克生物技术有限责任公司
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