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Cotton seed dregs hydrolysate and its preparation process and application

A technology of hydrolyzate and cottonseed meal is applied in the field of protein hydrolyzate to achieve the effect of inhibiting the germination of weed seeds

Inactive Publication Date: 2009-03-11
SHENZHEN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research and product development in this area have not yet been carried out, and the application of pre-emergent herbicides for weeds to the hydrolyzate prepared from natural protein feed cotton meal has not been reported yet.

Method used

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  • Cotton seed dregs hydrolysate and its preparation process and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] After adding deionized water to the cottonseed meal, shake it in a constant temperature oscillator at 60°C. After the temperature of the solution in the conical flask is constant, adjust the pH of the solution to 8.0 with 20% ammonia water to become a substrate. According to the mass ratio of cottonseed meal: deionized water =1:9; Add alkaline protease to the substrate for reaction, shake continuously during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 8.0, and the reaction time is 6h. Specific substrate: alkaline protease = 1:2; place the reaction solution in a water bath at 100°C and heat for 15 minutes to inactivate the enzyme, then centrifuge at 4000r / min for 20 minutes, take the supernatant, freeze it, and dry it into a dry powder. Refrigerate and set aside. Alkaline protease was selected from Alcalase 2.4L produced by Novo Nodisk Company in Denmark, with a calibration activity of ...

Embodiment 2

[0026] Similar to Example 1, the difference is that after adding deionized water to the cottonseed meal, shake in a constant temperature oscillator at 60°C, after the temperature of the solution in the conical flask is constant, adjust the pH of the solution to 7.8 with 20% ammonia water to become a substrate, According to the mass ratio of cottonseed meal: deionized water=1:8; add alkaline protease reaction in the substrate, shake constantly during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 7.8, the reaction time is 5 hours, according to the mass ratio of substrate: alkaline protease = 1:1; the reaction solution is placed in a 100°C water bath and heated for 10 minutes to kill the enzyme, then centrifuged at 3800r / min for 15 minutes, and the supernatant is frozen. Dried into dry powder, refrigerated for later use.

Embodiment 3

[0028] Similar to Example 1, the difference is that after adding deionized water to the cottonseed meal, it is shaken in a constant temperature oscillator at 60° C. After the temperature of the solution in the conical flask is constant, the pH of the solution is adjusted to 8.5 with 20% ammonia water to become a substrate. According to the mass ratio of cottonseed meal: deionized water=1:10; add alkaline protease reaction in the substrate, constantly shake in the reaction process, the temperature is kept at 60 ℃, and dropwise add 20% ammonia water to maintain the pH value of the reaction system at 8.5, the reaction time is 8h, according to the mass ratio of substrate: alkaline protease=1:3; the reaction solution is placed in a 100°C water bath and heated for 20min to kill the enzyme, then centrifuged at 4200r / min for 25min, and the supernatant is frozen. Dried into dry powder, refrigerated for later use.

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Abstract

The present invention relates to protein hydrolysate, and is especially cotton seed dregs hydrolysate and its preparation process and application. The cotton seed dregs hydrolysate is applied as herbicide for suppressing weed germination. It is yellow brown powder and water soluble, and has protein content more than 75 wt%, ash content less than 5 wt%, and pH 6.8. It is prepared through adding water into cotton seed dregs, vibrating, adding ammonia water to regulate pH value, adding alkali protease to react, adding more ammonia water to maintain pH value, deactivating enzyme in a water bath, centrifuging to obtain supernatant, and freeze drying to obtain powder.

Description

technical field [0001] The invention relates to a protein hydrolyzate, in particular to a hydrolyzate prepared from natural protein raw material cotton meal and its application in pre-emergence control of weeds. Background technique [0002] Herbicides have been widely used to control weeds in farmland, orchards and urban lawns. There are many types of herbicides, but most of them are chemical herbicides produced by chemical synthesis. Although chemical herbicides can control the growth and spread of weeds to a limited extent, their pollution to soil and groundwater is becoming increasingly apparent. At the same time, repeated use of chemical herbicides can easily lead to drug resistance of weeds. Therefore, it is an inevitable trend in the development of herbicides to produce natural herbicides without toxic and side effects. In recent years, people have successively discovered that some natural active peptides and proteolytic polypeptides in some organisms have herbicidal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02C12P1/00A01P13/02
Inventor 杨剑丁立忠杨少华
Owner SHENZHEN POLYTECHNIC