Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method

A technology of carotene and Torula rhodozyme, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low β-carotene content, achieve short growth cycle, high yield, temperature and Effects over a wide pH range

Inactive Publication Date: 2007-08-01
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently screened Rhodotorula strains have low β-caro

Method used

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  • Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method
  • Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0028] Example 1: Isolation and identification of β-carotene-producing Rhodotorulamucilaginosa CGMCC No. 1536

[0029] 1. Sample collection

[0030] Soil samples were collected from abandoned gardens in Urumqi, Xinjiang. After screening, optimization and other technical means, they were placed on a plate suitable for yeast medium, such as YPD medium (20% glucose, 20% peptone, 10% yeast extract), and separated at 28°C. The XJU-1 strain of Rhodotorulamucilaginosa was obtained.

[0031] 2. Bacteria identification

[0032] They were identified by molecular taxonomy based on the base sequence of the D1 / D2 region in the large subunit 26S rDNA and the base sequence analysis of the small subunit 5.8S rDNA and two internal transcribed spacers (ITS1 and ITS2). Combined with morphological observation and determination of physiological and biochemical properties, it was accurately identified as Rhodotorula mucilaginosa CGMCC No.1536.

Example Embodiment

[0033] Example 2: Cultivation of β-carotene-producing Rhodotorulamucilaginosa CGMCC No. 1536 strain and determination of fermentation process.

[0034] The selected medium is as follows:

[0035] ①Slant activation medium: glucose 2%; peptone 2%; yeast powder 1%; pH 9.0.

[0036] ②Liquid seed medium: glucose 2%; peptone 2%; yeast powder 1%; pH 8.5.

[0037] ③Liquid fermentation medium: glucose 4%; peptone 2%; yeast powder 1%; pH 8.0.

[0038] The present invention adopts conventional culture medium, but has special requirements for pH, and conventional fermentation is carried out in a slightly acidic environment, while the strain of the present invention can produce high beta-carotene in a slightly alkaline condition.

[0039] Fermentation culture method (referring to accompanying drawing 1):

[0040] ①Slant activation culture: connect the strain from the preservation slant to the activated slant medium, and cultivate at 28°C for 48h.

[0041] ②Liquid seed culture: connecte...

Example Embodiment

[0044] Example 3: Process for producing β-carotene by fermentation of Rhodotorula mucilaginosa CGMCC No.1536 strain.

[0045] The fermentation process steps in Example 2 were repeated, and the specific parameters in the fermentation process were repeatedly verified. The long-preserved strain of the present invention was inoculated into potato agar medium, under the conditions of pH 7.0, temperature 25° C., and shaking speed of 150 r / min, after culturing on a solid slant for 48.2 hours, fermentation was performed, and the fermentation broth was centrifuged (3000 rPmin) After 10 minutes, the supernatant was discarded, the precipitate was washed with water, and then centrifuged again. The obtained yeast mud was dried and weighed, and subjected to post-processing such as concentration and crystallization to obtain β-carotene, and the yield was 368 μg / g.

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Abstract

The invention relates to a viscous red torulin for beta- carotene production and the preparing method. The invention provides a viscous Rhodotorula mucilaginosa)CGMCC No.1536 for high- production of beta- carotene. The method comprises: activating viscous red torulin, fermentating for a certain of time, centrifuging and getting bacteria, disintegrating bacteria cell with thermal acid method, adding mixing liquid of petroleum ether and acetone with the proportion being 1: 1, oscillating at 28 Deg. C, extracting for one hour, and getting beta- carotene coarse extract. The invention is characterized by high productivity of beta- carotene, generally reaching 389 ug/ g, natural product of beta- carotene, no artificial colouring matter, and wide application of food and other industries.

Description

field of invention [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to a Rhodotorula yeast strain producing β-carotene, β-carotene and a production method thereof. Background technique [0002] β-carotene is an important natural pigment in plants, algae, fungi and other organisms, but it cannot be synthesized in humans and animals and must be ingested from the outside. At present, it is believed that β-carotene has anti-oxidation, anti-tumor, anti-aging effects, such as improving immunity for AIDS patients. In addition, β-carotene is the precursor of vitamin A, has the physiological activity of vitamin A, and can treat upper body cell keratinization, dry eye disease, and night blindness caused by vitamin A deficiency. (Yang Ning, Research Progress on Production of β-Carotene by Fermentation, Food Research and Development, 2004, 25(3): 19-21.) Abbreviation: FAO) and the World Health Organiza...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C07C403/24C12R1/88
Inventor 艾尔肯·热合曼杨艳艳易霞
Owner XINJIANG UNIVERSITY
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