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Method of separating and concentrating iridoid glycosides and crocin in cape jasmine fruit

A technology for iridoid glycosides and iridoid glycosides, which is applied in the field of separation and enrichment of iridoid glycosides components and crocin components, and can solve the problems of high toxicity, complicated purification steps, and comprehensive utilization of medicinal materials Low efficiency and other problems, to achieve the effect of reducing the cost of separation, high degree of comprehensive utilization, and improving the overall utilization rate

Inactive Publication Date: 2007-08-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the existing iridoid glycosides and crocin purification methods, such as high toxicity, complicated purification steps, and low comprehensive utilization rate of medicinal materials, and provide an environmentally friendly, simple and convenient operation, and high comprehensive utilization rate of medicinal materials. Method for separating and enriching iridoid glycoside components and crocin components from gardenia medicinal materials

Method used

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  • Method of separating and concentrating iridoid glycosides and crocin in cape jasmine fruit
  • Method of separating and concentrating iridoid glycosides and crocin in cape jasmine fruit

Examples

Experimental program
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Embodiment 1

[0021] Take 20 g of dried Gardenia fruit coarse powder, extract with 10 times the amount of 65% ethanol under reflux for 0.5 hour, and filter. The dregs were reflux extracted with 10 times the amount of 65% ethanol for 0.5 hours, centrifuged, filtered, and the filtrates were combined, concentrated under reduced pressure until there was no alcohol smell, and the volume was adjusted to 4 times the volume of the crude drug.

[0022] Put 120ml of purified AB-8 resin into a glass column with an inner diameter of 15mm and a height of 800mm. Add the 60ml gardenia extract prepared above. Wash away water-soluble impurities with 5 times the bed volume of pure water at a flow rate of 1 / 2 BV / h. Then 30% and 60% ethanol were used for elution at a flow rate of 1 / 2 BV / h, and the eluate was collected. Concentrate the above eluents under reduced pressure at 50°C, respectively, and dry them in vacuum at 60°C to obtain a yield of iridoid glycosides of 6.5%, of which the content of geniposide i...

Embodiment 2

[0024] Take 600g of dry gardenia fruit powder, reflux extraction with 10 times the amount of 65% ethanol for 3 times, the obtained extract is concentrated to about volume / crude drug ratio of about 4:1, centrifuged, and the supernatant is immediately mixed with 1 / 4 BV / The sample was loaded at a flow rate of h, washed with 5 times the bed volume, eluted with 30% ethanol, and the iridoid glycoside fraction was collected; then eluted with 60% ethanol and the saffron fraction was collected. The geniposide solution and the saffron solution were rotary evaporated below 60°C until viscous, and then baked in a vacuum oven to constant weight. The collected iridoid glycosides and saffron fractions were respectively concentrated and dried in vacuum to obtain 38.1 g of light yellow iridoid glycosides component powder and 26.5 g of reddish-brown crocins component powder. The yield of the iridoid glycoside component is 6.35%, wherein the geniposide content in the iridoid glycoside part is 6...

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Abstract

The invention discloses a separating method of enriched iridoid glycosides component and saffron component, which comprises the following steps: extracting condensed cape jasmine extract through alcohol; adsorbing through large-hole resin; eluting and enriching through alcohol gradiently; decompressing; condensing to obtain the product.

Description

technical field [0001] The invention relates to a method for enriching iridoid components and yellow pigment components, in particular to a method for separating and enriching iridoid glycoside components and saffron components from gardenia fruits. Background technique [0002] Gardenia was first recorded in "Shen Nong's Materia Medica" and listed as a middle grade. It is the dry ripe fruit of Rubiaceae Gardenia jasminoides Ellis. Mainly distributed in Jiangxi, Zhejiang, Hunan and Hubei and other regions. Harvest from September to November when the fruit is ripe and turns red and yellow. Bitter and cold in nature, returns to the Heart, Lung, and Sanjiao Meridians. It has the effects of purging fire and relieving restlessness, clearing away heat and diuresis, cooling blood and detoxifying. Indications for pyretic diseases, restlessness, jaundice, gonorrhea, thirst, red eyes, sore throat, hematemesis, epistaxis, bloody dysentery, hematuria, pyretic sores, sprains, swellin...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/04C07C51/42C07C57/13A61K36/744A61K131/00
Inventor 胡坪张敏罗国安王义明
Owner EAST CHINA UNIV OF SCI & TECH
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