Echinacea purpurea extractive and the preparing method and the content measuring method

A technology of echinacea extract and echinacea, applied in the field of medicine, can solve the problems of inconsistent quality standards of echinacea preparations, loss of polysaccharide content in the extract, different methods, etc., and achieves the effects of high sensitivity, simple steps and low cost

Active Publication Date: 2007-09-12
CHENGDU DIAO JIUHONG PHARMA FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the components used to control the quality standards of echinacea in various countries are different. For example, the United States adopts the content of caffeic acid components, Australia adopts caffeic acid compounds or alkanamide compounds, and in Europe, the main standard of echinacea products on the market is based on polysaccharides The content of Echinacea purpurea is determined, and there is no unified standard for Echinacea purpurea preparations in my country, which has caused the situation that the quality standards of Echinacea purpurea preparations are not uniform
The current research on the immune enhancement of Echinacea purpurea shows that the immune activity of Echinacea purpurea is not provided by any single component, but by the synergy of multiple components. Therefore, the extraction method of Echinacea genus plants has become particularly important. The methods are different. The content of various active ingredients in the extract is different, and even leads to the lack of certain types of ingredients. For example, echinacea is widely used at home and abroad to extract echinacea with ethanol. This method will inevitably lead to the loss of polysaccharide content in the extract. Publication No. The Chinese patent application CN 1473602A discloses a preparation method of Echinacea purpurea extract, that is, extracting twice with 30-60% ethanol under reflux, then filtering to remove impurities, concentrating and drying to obtain Echinacea purpurea extract

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] [Example 1] Preparation and content determination of Echinacea purpurea extract

[0034] Preparation: Take 10kg of Echinacea purpurea, pulverize, add 10 times the amount of 40% ethanol to reflux for extraction for 2 hours, filter, collect the filtrate, and recover the ethanol from the filtrate under reduced pressure to obtain extract (relative density is 1.06); use 10 times the amount of 20% ethanol Extract with ethanol for 1 hour, filter, collect the filtrate, and concentrate under reduced pressure to obtain an extract (relative density is 1.13); combine the obtained extracts, stir evenly, and spray dry to obtain the Echinacea purpurea extract.

[0035] Content determination: Take 50 mg of Echinacea purpurea extract, weigh it accurately, put it in a volumetric flask, add 25 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, take the The clear liquid...

Embodiment 2

[0036] [Example 2] Preparation and content determination of Echinacea angustifolia extract

[0037] Preparation: Take 10 kg of Echinacea angustifolia, crush it, add 12 times the amount of 60% ethanol to reflux for extraction for 1 hour, filter, collect the filtrate, and recover the ethanol from the filtrate under reduced pressure to obtain an extract (relative density is 1.07); use 12 times the amount of medicinal dregs extract with water for 1 hour, filter, collect the filtrate, and concentrate under reduced pressure to obtain an extract (relative density is 1.13); combine the obtained extracts, stir evenly, and spray dry to obtain the Echinacea purpurea extract.

[0038] Content determination: Take 50 mg of Echinacea angustifolia extract, weigh it accurately, put it in a volumetric flask, add 35 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, Get the s...

Embodiment 3

[0039] [Example 3] Preparation and content determination of Echinacea purpurea extract

[0040]Preparation: Take 10kg of Echinacea chinensis, pulverize, add 15 times the amount of 70% ethanol to reflux for extraction for 3 hours, filter, collect the filtrate, and recover ethanol from the filtrate under reduced pressure to obtain extract (relative density is 1.08); Extract with water for 1 hour, filter, collect the filtrate, concentrate under reduced pressure to obtain the extract (relative density is 1.15); combine the obtained extracts, stir evenly, and spray dry to obtain the extract of Echinacea purpurea.

[0041] Content determination: Take 50 mg of Echinacea purpurea extract, weigh it accurately, put it in a volumetric flask, add 40 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, and take The supernatant is used as the test solution; accurately weig...

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PUM

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Abstract

The present invention is one kind of Echinacea purpurea extract and its preparation process and content measuring method. The Echinacea purpurea extract is prepared through the following steps: extracting Echinacea purpurea material with 40-70 % concentration water solution of alcohol, filtering to obtain filtrate and dregs, concentrating the filtrate to obtain extractum; extracting the dregs with water or 10-30 % concentration water solution of alcohol, filtering to obtain filtrate and dregs, concentrating the filtrate to obtain extractum; merging extractum, and drying to obtain the Echinacea purpurea extract. The Echinacea purpurea extract has complete components, simple preparation process and low cost.

Description

technical field [0001] The invention relates to a plant extract and its preparation method and content determination method, in particular to an echinacea purpurea extract, its preparation method and content determination method, and belongs to the technical field of medicine. Background technique [0002] The genus Echinacea is a kind of Compositae plant native to America. As early as the 18th century, North American Indians first used Echinacea as a medicinal product. In the 19th century, North American immigrants also used it widely, and then gradually spread to Europe. There are three kinds of plants that are used medicinally, namely Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida, which are commonly used as upper respiratory tract infections and are used for wounds, insect bites and other diseases. Preparations are currently widely used immune enhancers in the world. (Liu Yibing. Chemistry, Immunity and Clinical Effects of Echinacea Plant Preparations...

Claims

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Application Information

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IPC IPC(8): A61K36/287A61P37/04G01N30/02G01N33/15
Inventor 刘瑜何民王晓凤白红艳刘忠荣及元乔李伯刚
Owner CHENGDU DIAO JIUHONG PHARMA FACTORY
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