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Gene chip and method of identifying highly effective ore leaching strain utilizing the same

A technology of gene chips and bacteria strains, which is applied in the field of rapid screening of highly efficient ore leaching strains, can solve the problems of heavy workload, poor reliability, and long time for screening of ore leaching strains

Inactive Publication Date: 2007-09-12
CENT SOUTH UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

And use the gene chip to provide a method for rapid and accurate identification of acidophilic Thiobacillus ferrooxidans high-efficiency strains from the genetic level, so as to solve the problems of heavy workload, long time and poor reliability in the screening of leaching strains

Method used

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  • Gene chip and method of identifying highly effective ore leaching strain utilizing the same

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Embodiment 1

[0015] Embodiment 1: Preparation of gene chip of the present invention

[0016] The present invention firstly constructs a whole-genome gene chip of type A.f strain ATCC 23270, which contains all 3217 genes of the strain. The genomic DNA of 5 strains of highly active A. f bacteria was hybridized with the constructed gene chip, and it was found that they jointly contained 320 genes related to ferrous and sulfur oxidation and resistance. The RNA of 5 highly active bacteria was further extracted and hybridized with the gene chip. Through the analysis of the gene expression map, it was found that a total of 135 genes were highly expressed in different highly active bacteria among the 320 characteristic genes, thus finding the genes directly related to the leaching performance. There are 135 functional genes, and the 135 genes are listed in Tables 1 to 4. They include 72 genes related to energy metabolism such as ferrous iron and sulfur oxidation, 18 genes related to high temperatu...

Embodiment 2

[0020] Embodiment 2: use the gene chip of the present invention to identify high-efficiency ore leaching bacterial strains

[0021] (1) Cultivation of the sample strain to be tested, DNA or RNA extraction and fluorescent labeling

[0022] Obtain a strain A.f bacterium (CMS05 bacterial strain) from certain copper ore isolation as the sample strain to be tested, carry out the expansion culture of sample, use 9K medium, every liter of medium contains 3g (NH 4 ) 2 SO 4 , 0.1g KCl, 0.5g K 2 HPO 4 , 0.5g MgSO 4 ·H 2 O, 0.01g Ca(NO 3 ) 2 , 44.3g FeSO 4 ·7H 2 O, 5g elemental sulfur, and add 2000g of lead acetate, 100g of silver nitrate, 0.4g of mercury sulfate, and 2500g of copper sulfate in the middle of the logarithmic growth period of the sample bacteria (the concentration of bacteria is not less than 107 / mL, and the total amount is not less than 2L). , Arsenic trioxide (As 2 o 3 ) 100 g, collect the bacterial liquid after culturing for 1 hour. DNA or RNA is extracted ...

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Abstract

The invention discloses a kind of gene chip and a method for identifying the efficient leaching bacterium with the chip. In the invention, it forms the gene chip with the 115~135 of functional genes directly related to the leaching property which are chose from the full-length gene strain ATCC23270. Comparing with the traditional method, the invention shortens the detection period from a few months to 3 ~ 5 days, and makes the results more accurate.

Description

technical field [0001] The invention relates to a gene chip and a method for screening microorganisms using the gene chip, in particular to a gene chip and a screening method for rapidly screening high-efficiency ore leaching bacterial strains from a large number of ore leaching microorganisms. Background technique [0002] Microbial metallurgy is a technology that uses the action of microorganisms as energy, decomposes minerals through microbial oxidation, makes metal ions enter the solution, and further separates and extracts metals. Microbial metallurgy has the characteristics of short process, low cost, environmental friendliness and low pollution. It can especially process low-grade, complex and difficult mineral resources. It is the cutting-edge science and technology of mineral extraction in the 21st century. The key to microbial metallurgy is to obtain excellent strains with high oxidation activity, strong specificity, and good environmental adaptability to improve t...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 邱冠周刘学端覃文庆刘新星申丽王军
Owner CENT SOUTH UNIV
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