Chitosan nanoparticles oral preparations of hepatitis vaccine

A technology for chitosan nanoparticles and oral preparations, which is applied in the field of medicine, can solve the problems of reduced bioavailability, no reports on oral preparations of hepatitis vaccine chitosan nanoparticles, and inability to achieve localization and uptake of nanoparticles. Narrow distribution, efficient triggering of immune response, and achieving the effect of oral vaccination

Inactive Publication Date: 2007-09-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the inability to realize the targeted uptake of nanoparticles, it is easy to reduce the bioava...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Materials and Reagents:

[0024] Chitosan Shanghai Ruji Biotechnology Development Co., Ltd. has a deacetylation degree of 90%, and an average molecular weight of 40-80kDa;

[0025] Sodium tripolyphosphate Sinopharm Chemical Reagent Co., Ltd. Lot number: F20050127 Chemically pure hepatitis B vaccine stock solution Beijing Tiantan Biological Products Co., Ltd.

[0026] Glacial acetic acid, Sinopharm Chemical Reagent Co., Ltd., analytically pure

[0027] 25% Glutaraldehyde Shanghai Pharmaceutical (Group) Shanghai Chemical Reagent Company 0.25g / 10ml

[0028] Lectins Sigma Reagents Inc.

[0029] Weigh 200mg of chitosan and dissolve in 100mL of 0.5% vinyl acetic acid to obtain a 0.2% chitosan solution. A 0.2% sodium polyphosphate solution was prepared with distilled water, and the hepatitis B vaccine stock solution was added thereto, and the concentration of the hepatitis B vaccine was 200 μg / ml. Under continuous stirring at a speed of 600 rpm, the sodium polyphosphate so...

Embodiment 2

[0032] The dosage of chitosan is 300mg, the concentration of sodium polyphosphate solution is 0.5%, and the dosage of glutaraldehyde is 1.2mL. All the other are the same as embodiment 1, particle diameter is 496.3nm.

Embodiment 3

[0034] The amount of chitosan was 100 mg, the concentration of sodium polyphosphate solution was 0.2%, the amount of glutaraldehyde was 0.5 mL, and the rest were the same as in Example 1, and the particle diameter was 330.5 nm.

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Abstract

The invention relates to medicine technique domain. At present, the hepatitis vaccine is in common use of protective inoculation of hepatitis. The current formulation is injection, with the shortcomings of high cost, poor tolerance and need specialized technical personnel administrating. The chitosan nanoparticles preparation is formed by ionic crosslinking-high pressure homogeneization technique through hepatitis vaccine model and chitosan as carrier material, furthermore the chitosan nanoparticles are surface modified by lectin to obtain nanoparticles preparation suitable for oral inoculation. The said nanoparticles preparation vaccine provides a new route of oral inoculation to prevent hepatitis B, with high operability and low cost.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an oral chitosan nanoparticle preparation of hepatitis vaccine. Background technique [0002] Viral hepatitis is a common and frequently-occurring disease that is distributed all over the world. Due to the obvious regional differences in health conditions and population immunity in China, the number of potential sources of infection is huge, it is difficult to prevent and control, and it has a wide range of hazards. The annual economic loss is huge. The cure rate for viral hepatitis is unsatisfactory, although a few medicines have certain curative effect, but side effect is big, cost is high. For viral hepatitis, prevention is the main focus. Hepatitis vaccine is currently a commonly used preventive method. However, vaccination is usually administered by injection, which has problems such as high production cost, poor tolerance, and the need for professional and technical person...

Claims

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Application Information

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IPC IPC(8): A61K39/29A61K47/36A61K9/00
Inventor 胡晋红费轶博李凤前王晶刘继勇
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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