Method for measuring content of 2 beta-[N, Níõ-bis (2-mercaptoethyl) ethylenediamino] methyl, 3 beta-(4-chlorophenyl) tropane in kit

A technology of ethylenediamine and medicine box, which is applied in the field of determination of TRODAT-1 content, can solve the problem of not measuring the content of TRODAT-1 in the medicine box, and achieve the effect of simple operation and good repeatability

Inactive Publication Date: 2007-09-26
JIANGSU INST OF NUCLEAR MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When performing quality control on the TRODAT-1 kit, the content of TRODAT-1 in the kit is a key indicator. At present, there are no reports on the determination of the content of TRODAT-1 in the kit at home and abroad

Method used

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  • Method for measuring content of 2 beta-[N, Níõ-bis (2-mercaptoethyl) ethylenediamino] methyl, 3 beta-(4-chlorophenyl) tropane in kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Acquisition of the standard curve equation: Accurately draw 40, 60, 100, 120, and 150 μL of the TRODAT-1 standard solution with a concentration of 1 mg / ml and place them in a 30 ml separating funnel, and add the following solutions in turn: 8.0 ml of chloroform, bromomuscarin 4.0ml of turf blue solution, 5.0ml of disodium hydrogen phosphate-citric acid buffer solution at pH 4.0, shake for 2 minutes, let stand to separate layers, separate the clear chloroform layer, dry it with anhydrous sodium sulfate Put in a 1cm cuvette, without adding TRODAT-1 standard solution, the chloroform solution prepared by the same steps as above was used as a blank, and the absorbance values ​​were measured at 414nm wavelength (as shown in Table 1). Perform linear regression calculation on the content C (μg) of TRODAT-1 with the absorbance value (A), and obtain the standard curve equation (regression equation) as: A=0.0049C+0.1143.

[0013] The standard curve made with the absorbance value o...

Embodiment 2

[0016] Determination of the content of TRODAT-1 in the kit: In three 30ml separating funnels, add the following solutions in turn: 8.0ml of chloroform, 4.0ml of bromothymol blue solution, take 3 different batches of TRODAT-1 2 pieces in each kit, 6 pieces in total, add 2.5ml of disodium hydrogen phosphate-citric acid buffer solution with pH 3.58 for each 2 pieces (same batch number), shake well to dissolve, transfer to the above separatory funnel, and then add pH 2.5ml of 3.58 disodium hydrogen phosphate-citric acid buffer solution, shake well to dissolve, transfer to the above separatory funnel, shake for 2 minutes, let stand to separate the layers, separate the clear chloroform layer, and use Put in the 1cm cuvette after sodium sulfate water drying, take the blank solution in Example 1 as a blank, measure the absorbance value respectively at 414nm wavelength place, and substitute the regression equation in Example 1 to calculate its content, 3 different batch numbers The kit...

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Abstract

This invention relates to one method to test the content of 2beta-[N, N'-double(2-thioglycollic base) diethylenediamino] methyl -3beta-(4- chlorphenyl) tropane in the test technique field of TRODAT-1. This invention also provides one method to test TRODAT-1 content in drug case for the measurement of the object with easy operations and good repeat ability.

Description

technical field [0001] A method for determining the content of 2β-[N,N'-bis(2-mercaptoethyl)ethylenediamine]methyl-3β-(4-chlorophenyl)tropane in a kit, involving TRODAT-1 Content determination technology field. Background technique [0002] Technetium [ 99m Tc]-2β-[N, N'-bis(2-mercaptoethyl)ethylenediamine]methyl-3β-(4-chlorophenyl)tropane ( 99m Tc-TRODAT-1) is the first technetium[ 99m Tc] labeled dopamine transporter imaging agent successfully used in the human body, it can specifically combine with dopamine transporter in the brain and concentrate in the striatum, providing clear images and prompting changes in the dopaminergic system. A large number of human studies at home and abroad have shown that: 99m Tc-TRODAT-1 is an imaging agent for single-photon emission computed tomography (SPECT) imaging, which has excellent clinical application potential for related diseases, especially early diagnosis of Parkinson's disease. This imaging agent will soon be available at h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 陈正平李晓敏王颂佩唐婕刘春仪陆春雄蒋泉福杨敏潘栋辉徐宇平
Owner JIANGSU INST OF NUCLEAR MEDICINE
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