Preparation of feeding live bacterial acid, and dedicated bacterial strain

A preparation and feeding technology, which is applied in the direction of bacteria, application, animal feed, etc., can solve the problems of large amount of addition, easy inactivation of live bacterial preparations, and inability to export, so as to improve feed conversion rate, reduce diarrhea rate of piglets, improve The effect of digestive function

Inactive Publication Date: 2007-10-10
北京君德同创生物技术股份有限公司
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Acidifiers and live bacteria preparations cannot completely replace feed antibiotics
The application effect of acidifier in pigs is remarkable, especially for weaned piglets. It can make up for the deficiency of gastric acid secretion, activate protease activity, and promote the digestion and absorption of nutrients. It can be used as the first choice for antibiotic substitutes. The need is large, and it is also greatly affected by the buffering capacity of the diet. In production practice, it is impossible to add a large dose of acidulant
As a substitute, probiotics have the disadvantages that live bacterial preparations are easy to inactivate, are not resistant to the acidic environment of the stomach, and are difficult to gain a competit...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1, the cultivation and safety detection of Bacillus subtilis subsp.subtilis Gendone-01 CGMCC No.1946

[0038] 1. Bacteria culture

[0039] The composition of the seed medium used in this embodiment is: every liter of culture solution contains: 5.0 grams of glucose, 5.0 grams of peptone, 3.0 grams of beef extract, 1.0 grams of yeast powder, MgSO 7H 2 O 0.5 g, MnSO4·H 2 O 0.005 g, pH 7.0.

[0040] Composition of fermentation medium (g / L): corn flour 3.0; soybean flour 6.0; peptone 2.0; glucose 3.0; urea 2.0; magnesium sulfate heptahydrate 0.5; manganese sulfate monohydrate 0.5; 7.5.

[0041] Pick 2 rings of the activated Bacillus subtilis subsp.subtilis Gendone-01 CGMCC No.1946 with an inoculation loop in the seed medium, and shake the shaker for 24 hours, then take 2% (volume ratio) ) The seed culture solution was transferred to a 5-ton fermenter with 3.6 tons of fermentation solution and cultivated for 36 hours. Among them, the culture temperature is 35-38°...

Embodiment 2

[0044] Embodiment 2, the preparation of live bacterial acid preparation for feeding

[0045] Feed viable bacterial acid preparation prepared with the feed viable bacteria agent prepared in Example 1, the composition of which is shown in Table 2. Wherein the content of Bacillus subtilis subsp.subtilis Gendone-01 CGMCCNo.1946 is 1.4×10 9 CFU / g live bacterial acid preparation for feeding.

[0046] raw material name

Proportion (kg)

Hydrated silica (excipient)

8

The live bacteria agent for feeding prepared by embodiment 1

14

Anhydrous Calcium Lactate

10

anhydrous citric acid

40

fumaric acid

5

malic acid

3

Lactose (as an excipient)

20

Total (kg)

100

[0047] Its preparation method is as follows:

[0048] The production capacity of each batch is 250 kg. First weigh 100 kg of anhydrous citric acid, 25 kg of anhydrous calcium lactate, 12.5 kg of fumaric acid, a...

Embodiment 3

[0049] Embodiment 3, animal experiments

[0050] The percentages in this animal experiment are all mass percentages.

[0051] 1. Experimental design

[0052] In this animal experiment, 72 weaned piglets (Duroc×Landrace×Large White) aged 21 days and with an average body weight of 5.3±0.1 kg were selected and divided into 4 treatments, 18 piglets in each treatment. The first group is the control group, fed only the basal diet a in Table 3; the second group is fed with live bacterial acid preparation group, fed with diet b; ; The fourth group is the strong acid spirit group, fed with diet d. 6 replicates per treatment, 3 pigs per replicate. The feeding period was 28 days.

[0053] Among them, the basic diet a is mainly corn, puffed soybean meal, and puffed soybean, and a small amount of fish meal, whey powder and glucose are added. The composition and nutrient level of the basal diet a are shown in Table 3. On the basis of the basal ration a, reduce the amount of corn, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention discloses a feed-use living bacterium acidic preparation and its special strain. The active component of the feed-use living bacterium acidic preparation is Bacillus subtilis subsp. Subtilis Gendone-01 (CGMCC No.1946). The feed-use living bacterium acidic preparation can be used for livestock as a substitute for antibiotics. And the livestock product does not have residual antibiotics, which is good for human health. Besides, the feed-use living bacterium acidic preparation can increase feed utility, reduce feed cost, sterilize in intestines, reduce diarrhea, and improve anti-stress ability and immunity.

Description

technical field [0001] The invention relates to a live bacterial acid preparation for feeding and a special bacterial strain thereof. Background technique [0002] With the improvement of people's living standards and the strengthening of environmental protection awareness, the requirements for the quality of animal products and the hygienic environment for animal feeding are getting higher and higher. Traditional feed antibiotic additives are gradually being banned or restricted. In January 2006, the European Union It has been formally decided to ban the addition of growth-promoting antibiotics to livestock and poultry feed. Subsequently, Japan, South Korea and other Asian developed countries also formulated corresponding policies and regulations to limit antibiotic residues in animal products. [0003] Finding alternatives to antibiotics and alleviating the negative impact of antibiotic ban on livestock and poultry production is an urgent problem to be solved by the anima...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A23K1/16C12R1/125A23K10/18A23K20/10
Inventor 朱元招张德福尚秀国杨立彬陆文清石凤云王长彦生广旭张蓝庭于维军苏振刚
Owner 北京君德同创生物技术股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap