Preparation of feeding live bacterial acid, and dedicated bacterial strain
A preparation and feeding technology, which is applied in the direction of bacteria, application, animal feed, etc., can solve the problems of large amount of addition, easy inactivation of live bacterial preparations, and inability to export, so as to improve feed conversion rate, reduce diarrhea rate of piglets, improve The effect of digestive function
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0037] Example 1, the cultivation and safety detection of Bacillus subtilis subsp.subtilis Gendone-01 CGMCC No.1946
[0038] 1. Bacteria culture
[0039] The composition of the seed medium used in this embodiment is: every liter of culture solution contains: 5.0 grams of glucose, 5.0 grams of peptone, 3.0 grams of beef extract, 1.0 grams of yeast powder, MgSO 7H 2 O 0.5 g, MnSO4·H 2 O 0.005 g, pH 7.0.
[0040] Composition of fermentation medium (g / L): corn flour 3.0; soybean flour 6.0; peptone 2.0; glucose 3.0; urea 2.0; magnesium sulfate heptahydrate 0.5; manganese sulfate monohydrate 0.5; 7.5.
[0041] Pick 2 rings of the activated Bacillus subtilis subsp.subtilis Gendone-01 CGMCC No.1946 with an inoculation loop in the seed medium, and shake the shaker for 24 hours, then take 2% (volume ratio) ) The seed culture solution was transferred to a 5-ton fermenter with 3.6 tons of fermentation solution and cultivated for 36 hours. Among them, the culture temperature is 35-38°...
Embodiment 2
[0044] Embodiment 2, the preparation of live bacterial acid preparation for feeding
[0045] Feed viable bacterial acid preparation prepared with the feed viable bacteria agent prepared in Example 1, the composition of which is shown in Table 2. Wherein the content of Bacillus subtilis subsp.subtilis Gendone-01 CGMCCNo.1946 is 1.4×10 9 CFU / g live bacterial acid preparation for feeding.
[0046] raw material name
Proportion (kg)
8
The live bacteria agent for feeding prepared by embodiment 1
14
10
40
5
3
20
Total (kg)
100
[0047] Its preparation method is as follows:
[0048] The production capacity of each batch is 250 kg. First weigh 100 kg of anhydrous citric acid, 25 kg of anhydrous calcium lactate, 12.5 kg of fumaric acid, a...
Embodiment 3
[0049] Embodiment 3, animal experiments
[0050] The percentages in this animal experiment are all mass percentages.
[0051] 1. Experimental design
[0052] In this animal experiment, 72 weaned piglets (Duroc×Landrace×Large White) aged 21 days and with an average body weight of 5.3±0.1 kg were selected and divided into 4 treatments, 18 piglets in each treatment. The first group is the control group, fed only the basal diet a in Table 3; the second group is fed with live bacterial acid preparation group, fed with diet b; ; The fourth group is the strong acid spirit group, fed with diet d. 6 replicates per treatment, 3 pigs per replicate. The feeding period was 28 days.
[0053] Among them, the basic diet a is mainly corn, puffed soybean meal, and puffed soybean, and a small amount of fish meal, whey powder and glucose are added. The composition and nutrient level of the basal diet a are shown in Table 3. On the basis of the basal ration a, reduce the amount of corn, and ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com