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The coupling substrate between magnetic resonance imaging contrast agent based on metal fullerene and antibody and the detecting technology

A metallofullerene, magnetic resonance imaging technology, applied in the detection technology of whether the antibody is active in the above-mentioned coupling, in the field of coupling of magnetic resonance imaging contrast agent and antibody, to avoid aggregation or even precipitation, and avoid separation process , the effect of high sensitivity

Active Publication Date: 2007-10-31
赤峰福纳康生物技术有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on MRI contrast agents with targeted detection function based on metallofullerenes has not been reported in the literature.

Method used

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  • The coupling substrate between magnetic resonance imaging contrast agent based on metal fullerene and antibody and the detecting technology
  • The coupling substrate between magnetic resonance imaging contrast agent based on metal fullerene and antibody and the detecting technology
  • The coupling substrate between magnetic resonance imaging contrast agent based on metal fullerene and antibody and the detecting technology

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Experimental program
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Effect test

Embodiment 1

[0048] (1) Prepare 0.1 oz. M's 1-ethyl-3 (3-dimethyl-amino-pentyl) carbodiimide hydrochloride (EDC) aqueous solution;

[0049] (2)Gd@C 82 o 6 (OH) 16 (NHCH 2 CH 2 COOH) 8 preparation of

[0050] Prepare a 3mol / L NaOH solution of β-alanine, and add ethanol twice the volume of the alkaline solution; at 25°C, add the solution dropwise to the toluene solution (~5mg) of metallofullerenes, continue Stir the reaction for 3 days, separate and concentrate, use Sephadex as the stationary phase, deionized water as the mobile phase, collect the components with pH=6-7, and obtain the target product-a water-soluble derivative of metallofullerene, Gd @C 82 o m (OH) n (NHCH 2 CH 2 COOH) l Where m=6, n=16, l=8 (AAD-EMFs for short).

[0051] (3) Preparation of conjugates

[0052] Carboxyl-containing water-soluble metallofullerene derivatives, Gd@C 82 o 6 (OH) 16 (NHCH 2 CH 2 COOH) 8 , (40μL, 2×10 -7 M), activated with fresh aqueous EDC (10 μL, 0.1 M) for 15 min. At room t...

Embodiment 2

[0054] (1) with embodiment 1 (1)

[0055] (2)Gd@C 60 [(C(COOH) 2 )] 10 preparation of

[0056] In Gd@C 60 (~10mg) THF solution was added relative to Gd@C 60 20 times molar equivalent of diethylbromomalonate and excess NaH, stirred at 20°C for 2h, Gd@C 60 It is rapidly transformed into malonate derivatives. Add a hydrochloric acid solution with a concentration of 60wt% to reflux for 12h, Gd@C 60 The malonate derivatives are transformed into water-soluble malonate derivatives. Stand still, separate the liquid, and concentrate the water phase under reduced pressure, then use Sephadex G-25 as the stationary phase, use deionized water as the eluent, and collect the brown fraction with pH = 6-7, which is used in the present invention Gd@C 60 The carboxylated water-soluble derivative of Gd@C 60 [C(COOH) 2 ] 10 .

[0057] (3) Preparation of conjugates

[0058] Purified water-soluble derivatives of Gd-containing metallofullerenes containing carboxyl groups with contrast e...

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Abstract

The invention discloses a magnetic resonance image contrast agent based on metallic enriched ocimene and coupling matter of antibody, which comprises the following steps: (a) choosing water-soluble metallic enriched ocimene derivant with carboxy group; activating with fresh 1-ethyl group-3 (3-dimethyl-amido-amyl group) carbodiimide alcaine salt water solution; (b) adding antibody into the solution of the step (a); setting the molar weight of the antibody at 1 / 10-1 / 20 of the water-soluble metallic enriched ocimene derivant; stirring; reacting; getting the product. This invention also discloses a testing technique of the antibody. This invention possesses simple course and warm reacting condition, which possesses larger prospect.

Description

technical field [0001] The invention belongs to the field of magnetic resonance imaging contrast agents, and relates to a metallofullerene-based coupling of magnetic resonance imaging contrast agents and antibodies. The present invention also relates to a technology for detecting whether the antibody in the above-mentioned conjugate has activity. Background technique [0002] Magnetic resonance imaging (MRI) technology has now developed into a common method in clinical medical diagnosis, and it is one of the most effective methods for diagnosing tumors. In order to enhance the contrast and clarity between diseased tissue and normal tissue images, it is necessary to select an appropriate contrast enhancer to reveal anatomical features. Most of these contrast enhancement agents utilize the paramagnetic properties of metal ions. Gd 3+ Because of its strong paramagnetism, its complexes are currently the most widely used and most effective class of contrast enhancement agents....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/06
Inventor 王春儒舒春英马新勇方晓红
Owner 赤峰福纳康生物技术有限公司
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