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Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof

A colorless bacillus and arsenic-polluted technology, applied in the field of environmental microorganisms, to achieve the effect of easy to harm the environment, strong mobility, and reduce the harm of arsenic

Inactive Publication Date: 2007-10-31
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the existing environmental arsenic pollution purification technology, and obtain a new strain of arsenic oxidizing bacteria, which can oxidize highly toxic trivalent arsenic in the environment to low toxic arsenic pollution

Method used

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  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof
  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof
  • Xylose oxidation achromatous bacillus SY8 for purifying arsenic contamination and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Isolation of Achromobacter xyloseoxidizing SY8 from arsenic-contaminated soil

[0024] The experimental soil was taken from arsenic-contaminated topsoil in Shayang County, Hubei Province, China. The specific operation steps are as follows:

[0025] (1) Sample enrichment: Accurately weigh 100g of soil sample into a 250ml sterilized conical flask, add 5ml of 80mM NaAsO 2 , Gently stir well and place in a 28°C incubator for one week, pay attention to adding sterile water to ensure that the samples are not dry.

[0026] (2) Isolation of arsenic-resistant bacteria: accurately weigh NaAsO 2 10g of enriched soil samples were placed in a triangular flask containing 90ml of sterile physiological saline, shaken in a shaker at 28°C for half an hour, and then 1ml to 9ml of sterile physiological saline was gradually diluted to 10 -3 , 10 -4 , 10 -5 , respectively take 0.1ml of coating containing 800uM NaAsO 2 The CDM solid medium plates of 100% were coated with 3 pla...

Embodiment 2

[0030] Example 2: Arsenic oxidation curve of Achromobacter xylose oxidizing SY8

[0031] Pick Achromobacter xylose oxidizing bacteria SY8 monoclone and inoculate it into 100ml CDM liquid medium, and place it in a shaker at 28°C for 48 hours of shaking culture. At this time, the cell density is OD. 600 is about 0.15, stored in a refrigerator at 4°C, and used as a seed bacterial solution for inoculation. Pipette 1ml into 100ml of fresh CDM liquid medium at 1% inoculum volume, place it in a shaker at 28°C for 12 hours and then add NaAsO 2 The mother liquor was made to have a final concentration of 800 uM, and after culturing for 9 hours, sampling was started, and samples were taken every 1 hour until NaAsO 2 Until complete oxidation, take 1.2ml and 0.6ml each time, respectively, and then measure the cell density (OD) by spectrophotometry. 600 ) and pentavalent arsenic concentration (OD 838 ), all cash out. The specific methods are as follows: First, measure the cell density (...

Embodiment 3

[0032] Example 3: The oxidation effect of Achromobacter xylose oxidizing bacteria SY8 on trivalent arsenic in simulated arsenic-polluted water

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Abstract

The invention discloses a wood sugar oxidation achromobacter SY8 and appliance in purifying arsenic contamination aspect in environmental microbiology technical domain, which is characterized by the following: separating a wood sugar oxidation achromobacter SY8 from arsenic contamination soil; possessing oxidation for inorganic tervalence arsenic; oxidizing inorganic tervalence arsenic to inorganic pentavalent arsenic in arsenic environmental pollution; decreasing toxicity of arsenic in environment; decreasing adsorbing removing property of arsenic; setting the preserved number as CCTCC NO: M207048. This strain possesses better applying prospect in arsenic pollution aspect.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to the screening of Achromobacter xylose-oxidizing SY8 which has an oxidizing effect on inorganic trivalent arsenic and its use in purifying arsenic pollution. Background technique [0002] At present, heavy metal pollution has become a global problem, especially arsenic pollution. World Health Organization officials announced that at least more than 50 million people in the world are facing the threat of endemic arsenic poisoning, most of them are Asian countries, and China is also one of the countries most seriously affected by arsenic poisoning. Although arsenic pollution in China has geological factors, human factors are more obvious. For example, mining and smelting of mines, processing of arsenic products, and coal burning have seriously polluted soil, groundwater and rivers. [0003] In response to arsen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F1/58B09C1/10A62D3/00C12R1/025
Inventor 蔡林王革娇
Owner HUAZHONG AGRI UNIV
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