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Method for preparing functional protein peptide

A functional protein and protease technology, applied in protein food processing, protein food ingredients, food science, etc., can solve the problems of low enzymatic hydrolysis efficiency, low degree of hydrolysis of cottonseed protein, long reaction time, etc., and achieve protein and arginine The effect of high content, low cellulose content, and low gossypol residue

Inactive Publication Date: 2007-10-31
中发麦迪尔生物科技江苏有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing functional protein peptides, which mainly solves the problems of using cottonseed as a suitable raw material for preparing functional protein peptides, reducing gossypol, anti-nutritional factor residues, and improving protein peptide yields; Solve the problems of low hydrolysis degree, long reaction time and low enzymolysis efficiency of cottonseed protein produced by traditional methods

Method used

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  • Method for preparing functional protein peptide

Examples

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Embodiment Construction

[0024] See Figures 1 and 2 for the examples, the method steps for preparing functional protein peptides:

[0025] Step 1: Using matured dephenolized cottonseed protein as raw material, firstly extract the cottonseed oil from the pretreated cottonseed with 6# solvent oil, remove gossypol with methanol, and then dry and mature the material at 100°C to 120°C for 10 -30 minutes, and then obtained as the raw material of the enzymatic protein peptide.

[0026] Step 2, combined hydrolysis with bromelain and neutral protease. First put the dried material into the enzymolysis reaction tank, stir and soak, first add bromelain, the addition amount is 3000 units / g raw material, after enzymolysis for 1 hour, add A.S1398 neutral protease, the addition amount is 4500 units / gram of raw material for joint hydrolysis. The temperature is 40-50°C, and the reaction time is 2-7 hours. During the hydrolysis process, the pH value on-line control system is used to dynamically control the pH of the r...

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Abstract

The invention discloses a preparing method of functionality protein peptide, which comprises the following steps: pretreating cotton seed; leaching out cotton oil with 6# solvent oil; stripping gossypol with carbinol; drying the material under 100-120 deg. c; ripening for 10-30 min; getting raw material; putting into enzymolysis retort; adding into bromelain; proceeding joint hydrolysis with A. S1398 neutral proteinase; controlling stirring speed; dropping progressively; proceeding dynamic state controlling monitor for acidity of material in enzymolysis retort; stabilizing pH value of the material at 7. 5-8. 5; filtering protein slurry in centrifuger; drying in spray drying tower; packing; getting the product. This invention can further improve enzymolysis efficiency and quality of protein peptide product.

Description

(1) Technical field [0001] The invention relates to a method for preparing functional protein peptides, in particular to a method for preparing functional protein peptides by enzymolysis using cottonseed as raw material. (2) Background technology [0002] Protein is a macromolecular substance, which is not easily digested and absorbed by the human body. Therefore, people often use hydrolysis to degrade it into small molecular substances such as peptone, peptide, and amino acid. Not only are they easy to absorb, but short peptides also have some special effects. Cottonseed has a high protein content and is a high-quality protein. However, cottonseed contains gossypol, which is harmful to the human body. The cake after cottonseed oil extraction is often directly processed into feed, and its rich protein has not been well utilized. There are two existing methods of using cottonseed to prepare functional protein peptides. One is to use high-temperature pre-pressed leaching and d...

Claims

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Application Information

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IPC IPC(8): C12P21/02A23J3/34
Inventor 焦洋李贤永孙猛白卫华
Owner 中发麦迪尔生物科技江苏有限公司
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