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Pharmacogenetic detection chip for cardiac and cerebral vascular diseases and its application

A technology for cardiovascular and cerebrovascular diseases and cerebrovascular diseases is applied in the field of pharmacogenetic detection chips for cardiovascular and cerebrovascular diseases, which can solve the problems of difficult personalized medicine and low throughput of detection methods.

Inactive Publication Date: 2007-11-07
SHANGHAI BIOCHIP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high polymorphism of genes related to cardiovascular and cerebrovascular diseases, the genetic variation involved in each gene ranges from tens to hundreds. Obviously, the traditional detection method is difficult to apply to personalized medicine due to the low throughput level.

Method used

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  • Pharmacogenetic detection chip for cardiac and cerebral vascular diseases and its application
  • Pharmacogenetic detection chip for cardiac and cerebral vascular diseases and its application
  • Pharmacogenetic detection chip for cardiac and cerebral vascular diseases and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1 Reverse hybridization

[0081] 1. Preparation of gene chip

[0082] (1) Probe dissolution

[0083] Each probe of the sequence shown in SEQ ID NO: 1 to SEQ ID NO: 54 was diluted with TE solution, and the final concentration was 10 mM. Mix the probe with a concentration of 10mM and the PBS solution with a concentration of 200mM in a medium volume of a 384-well plate, seal the 384-well plate with an adhesive sheet, shake at room temperature for 2 minutes, centrifuge, and store at -20°C for sample application .

[0084] (2) Spotting

[0085] The pre-designed and synthesized probes are loaded onto the solid-phase carrier substrates made of glass slides, silicon wafers, etc. through contact spotting or inkjet spotting. The film base adopts Cell Associates CSS-100 aldehyde base film base, (the Ominigrid 100 model spotting instrument of GeneMachine Company, at humidity: 65-75% (based on FullMoon film base), and the temperature is 25 °C. , After the sampling is co...

Embodiment 2

[0098] Example 2 Forward hybridization

[0099] 1. Amplification and microarray preparation of pharmacogenetic related genes of cardiovascular and cerebrovascular diseases

[0100] ACE primers (SEQ ID NO: 55, 56), AGT primers (SEQ ID NO: 57, 58), AT1R primers (SEQ ID NO: 59, 60), CYP11B2 primers (SEQ ID NO: 61, 62), primers for ADRB1 (SEQ ID NO: 63-66), primers for ADRB2 (SEQ ID NO: 67, 68), primers for GNB3 (SEQ ID NO: 69, 70), primers for eNOS (SEQ ID NO: 71, 72), primers for ADD1 (SEQ ID NO: 73, 74), primers for IRS1 (SEQ ID NO: 75, 76) and primers for TSC (SEQ ID NO: 77-80) to amplify genes. PCR amplification was carried out with 100μl reaction system, the reaction system was 0.3mM dNTP, 10mM Tris-HCl, 50mM KCl, 2mM MgCl 2 , 20% Q solution (Qiagen), 0.01 μM SHV-F, 0.2 μM SHV-R, 100 ng genomic DNA, 3U Ex Taq enzyme (Takara). Cycle parameters: denaturation at 94°C for 5 min; denaturation at 94°C for 40 s, annealing at 65°C for 1 min, extension at 72°C for 1 min, a total o...

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Abstract

The present invention relates to gene polymorphism detecting chip, and discloses one kind of pharmacogenetic detecting chip for cardiac and cerebral vascular diseases and comprising solid carrier and probe. The probe can hybridize with the nucleotide sequence and / or its complementary sequence of the gene related to cardiac and cerebral vascular disease pharmacogenetics. The present invention also discloses the method of using the chip in detecting the gene related to cardiac and cerebral vascular disease pharmacogenetics. The detecting chip of the present invention makes it possible for the medical staff to obtain cardiac and cerebral vascular disease pharmacogenetics of the patient for predicting clinical medicine treatment and individualized treatment.

Description

technical field [0001] The invention relates to a gene polymorphism detection chip, in particular to a pharmacogenetic detection chip for cardiovascular and cerebrovascular diseases and its application. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one disease that threatens middle-aged and elderly people. With its high incidence, high disability rate and high mortality rate as the most typical three high diseases, it has brought great harm to patients, families and society. Epidemiological surveys show that the average incidence of cardiovascular and cerebrovascular diseases in the world population is 10-20%; the number of people who die from cardiovascular and cerebrovascular diseases accounts for 52% of the death toll every year in the world, and is the number one killer of death. With the improvement of people's life, the change of diet structure and ecological environment, the number of patients with cardiovascular and cerebrov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 盛海辉姜潇占伊扬肖华胜
Owner SHANGHAI BIOCHIP
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