Efficient liquid-phase chromatography method for determing reserpine content in rauvolfia plants

Abstract

The invention provides a high-performance liquid chromatography method to determine reserpine quantity in rauvolfia genus. Characteristics: (1) column 18 to carbon alkyl bonded to silica fillers; (2) mobile phase is mixed solventcomposed of acetonitrile, methanol, 0.01% triethylamine, the volume ratio is acetonitrile 45% - 35%, methanol 10% - 25% triethylamine aqueous 45% - 40%, (3) detection wavelength 268 nm, column temperature was 40 deg.C , flow rate 1 ml / min, the sample size of 20 ul. The invention has been system comparative studied, and establish the high-performance liquid chromatography method to determine reserpine quantity in rauvolfia genus for the first time,which is accurate, sensitive, specific, simple and rapid analysis, etc.

Description

1. Technical field

[0001] The invention relates to a chromatographic analysis method, and specifically claims to be a high-performance liquid chromatographic method for determining the content of the active ingredient reserpine in plants of the genus Rauwolfia. 2. Background technology

[0002] Reserpine is extracted from the roots of Rauvolfia plants in the family Apocynaceae. It is a natural medicine with hypotensive effect. The main preparations include "Reserpine Tablets", "Reserpine Injection", " Compound Jiangya Tablets", etc., the structural formula of reserpine is as follows:

[0003] rserpine reserpine

[0004] Due to the complex structure and high cost of chemical synthesis, reserpine is currently extracted from the plants of the genus Rauwolfia, and the main resources are R.yunnanensis and R.vomitoria. As resources are depleted due to years of digging, artificial cultivation is on the rise. There are few studies on the content analysis of reserpine in Rauwolf...

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