Construction method of expression profile for high homologous degree polygene family

Abstract

The invention discloses a constructing method of expression spectrum of each member gene in the high-homologization gene family, which is characterized by the following: connecting RT-PCR random clone and fluorescent real-time quantitative PCR; using RT-PCR random clone to obtain expression condition of each member gene and relative abundance of the expressed gene; utilizing real-time fluorescent quantitative PCR to obtain the total expression quantity of each gene family; making the expression spectrum as new method to study the expression modulation of multi-gene family; providing theoretical guide of gene engineering modification for gliadin content of forage crop.

Description

technical field

[0001] The invention relates to a method for constructing expression profiles of multigene families, in particular to a method for constructing expression profiles of multigene families with high degree of homology. Background technique

[0002] A multigene family is a group of genes that originated from a common ancestor and thus have the same function and similar DNA sequences. Among the genes of Escherichia coli (E.coli), the known gene family members exceed 50% (Koonin et al., 1998), and this proportion may be even higher in eukaryotes (Chervitz et al., 1998 ; Semple and Wolfe, 1999). In plant genomes, multigene families are more of a feature. For example, 66% of the genes in Arabidopsis thaliana (A. thaliana) belong to gene families, and half of the families have at least five member genes (Analysis of the genome sequence of the flowering plant Arabidopsis thaliana, 2000), which is not the case in other ubiquitous in plants. Studying all the genes in...

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