Method for extracting high pure rebaudioside A from stevia sugar

A stevioside and recrystallization technology, which is applied in the field of rebaudioside A and natural high-efficiency sweeteners, can solve problems such as bad aftertaste, and achieve the effect of pure taste, food safety and pure sweetness

Inactive Publication Date: 2008-01-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Stevioside is 200 times sweeter than sucrose, but it has a certain unpleasant aftertaste

Method used

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  • Method for extracting high pure rebaudioside A from stevia sugar
  • Method for extracting high pure rebaudioside A from stevia sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 5L of mixed solution of methanol and isopropanol (99+1) to dissolve 1kg of raw material stevioside (glycoside mixture); place crystallization at -18°C for 24h, then transfer to 4°C for 48h; filter to obtain crystals of about 0.5 kg; recrystallize once under the above conditions, and obtain about 0.25kg of recrystallized crystals; dissolve the crystals with 20L of pure water, separate and concentrate with a membrane with a molecular weight cut-off of 500; dry at 0.08MPa vacuum below 70°C for 24h to obtain high-purity Reiba Di A glucoside product is about 0.2kg.

[0014] The liquid chromatograms of the rebaudioside A standard product (a) and the process product (b) are shown in Figure 1.

[0015] High performance liquid chromatography conditions:

[0016] Agilent 100 high performance liquid chromatography; 210nm ultraviolet detector;

[0017] Chromatographic column: Zorbaxsb C18 column (4.6×150mm); column temperature: 30°C;

[0018] Mobile phase: acetonitrile-wate...

Embodiment 2

[0021] Take 6L of mixed solution of methanol and isopropanol (99+1) to dissolve 1kg of raw material stevioside (glycoside mixture); place crystallization at -18°C for 48h, then transfer to 4°C for 24h; filter to obtain crystals of about 0.5 kg; recrystallize once under the above conditions to obtain about 0.25kg of recrystallized crystals; dissolve the crystals with 15L of pure water, separate and concentrate with a membrane with a molecular weight cut-off of 500; dry at 0.08MPa vacuum below 70°C for 16h to obtain high-purity Reiba Di A glycoside product 0.2kg.

Embodiment 3

[0023] Take 8L of mixed solution of methanol and isopropanol (99+1) to dissolve 1kg of raw material stevioside (glycoside mixture); place crystallization at -18°C for 48h, then transfer to 4°C for 48h; filter to obtain crystals of about 0.5 kg; recrystallize once under the above conditions to obtain about 0.25kg of recrystallized crystals; dissolve the crystals in 10L of pure water, separate and concentrate with a membrane with a molecular weight cut-off of 500; dry at 0.08MPa under vacuum for 12h at 70°C to obtain high-purity Reiba Di A glucoside product is about 0.2kg.

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Abstract

A method for extracting high-purity laibaody A glycoside from sweet synanthrin is carried out by taking sweet synanthrin containing laibaody A glycoside 30% as raw material, dissolving sweet synanthrin by mixed solvent of methanol and isopropyl alcohol, crystallizing at -18 deg. C for 24-48 hrs, crystallizing at 4 deg. C for 24-48 hrs, filtering to obtain crystal, re-crystallizing to obtain re-crystallizing crystal, dissolving by pure water 10-20L, separating by film with trapping molecular weight 500, concentrating, and drying below 70 deg. C at 0.08 MPa vacuum for 12-24 hrs to obtain final product. The water content is 3.48%, gray content is 0.26%, laibody A glycoside content is more than 90%, and sweet flavor is as 300 times as cane sugar. It tastes pure.

Description

technical field [0001] The invention discloses a method for extracting high-purity rebaudioside A from stevioside, which belongs to the technical field of food additives. The invention relates to a production method of a natural high-efficiency sweetener in the field of food additives, in particular to a method for extracting rebaudioside A with a purity greater than 90% from stevioside. Background technique [0002] Stevia is a natural sweetener extracted from the leaves of Stevia rebaudiana. It has the characteristics of high sweetness and low calorie. It is a natural sweetener with development value and health promotion after cane sugar and beet sugar. It is known as "the third sucrose in the world" internationally. my country is the main producer of stevia in the world, with an annual output of 700-800 tons. Stevioside can be used as a sucrose substitute to replace some artificial sweeteners such as cyclamate and saccharin. It is widely used in food, beverage and other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/20A23L1/236A23L27/30
Inventor 汤坚刘杰
Owner JIANGNAN UNIV
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