Method for preparing water-insoluble beta-1,3/1,6-dextran

A water-insoluble, glucan technology, applied in fermentation and other directions, can solve the problems of low β-glucan content and high β-glucan, and achieve the effect of simple operation and high yield

Inactive Publication Date: 2008-02-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main disadvantage of acid extraction is that the content of β-glucan in the extraction product is also low, because more β-glucan is hydrolyzed (degraded) by acid during the extraction process

Method used

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  • Method for preparing water-insoluble beta-1,3/1,6-dextran
  • Method for preparing water-insoluble beta-1,3/1,6-dextran

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1. Preparation of water-insoluble β-1,3 / 1,6-glucan and its by-product mannan

[0034] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0035] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0036]1. Enzymatic hydrolysis of yeast cell walls: Mix 100g of yeast cell walls (purchased from Hubei Yichang Angel Yeast Co., Ltd.), 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, at 37°C Stir with a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory, Puma brand adjustable speed stirrer, 200-300 rpm) for 30 minutes, and then at 3000 rpm (Shanghai Anting Scientific Instrument Factory) , DL6000B refrigerated centrifuge, the same below) centrifuged for 20 minutes to remove the supernatant to obtain 95.3g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0037] 2. Alkali treatment: 95.3 g of the product obtained in ...

Embodiment 2

[0059] Example 2. Preparation of water-insoluble β-1,3 / 1,6-glucan and its by-product mannan

[0060] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0061] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0062] 1. Enzymolysis of yeast cell wall: Mix 50g of yeast cell wall, 100mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 250ml of water, and use a constant speed stirrer at a medium speed at 35°C (Shanghai Specimen Model Factory) , Huma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 60 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 25 minutes. Cleared to obtain 47.80g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0063] 2. Alkali treatment: 47.80 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall is added to 500 ml o...

Embodiment 3

[0079] Example 3. Preparation of water-insoluble β-1,3 / 1,6-glucan and its by-product mannan

[0080] 1. Preparation of water-insoluble β-1,3 / 1,6-glucan

[0081] The production process of water-insoluble β-1,3 / 1,6-glucan is shown in Figure 1, and the specific steps are as follows:

[0082] 1. Enzymolysis of yeast cell wall: Mix 100g of yeast cell wall, 300mg (20000U / mg) lysozyme (derived from ovalbumin, Cayman) and 500ml of water, and use a constant speed stirrer at a medium speed (Shanghai Specimen Model Factory) at 40°C , Puma brand speed-adjustable agitator, 200-300 rpm) stirring, stirring time is 30 minutes, then centrifuged at 3000 rpm (Shanghai Anting Scientific Instrument Factory, DL6000B refrigerated centrifuge) for 20 minutes. Cleared to obtain 95.4g of precipitate, which is the product of yeast cell wall enzymatic hydrolysis.

[0083] 2. Alkali treatment: 95.4 g of the product obtained in step 1 after enzymatic hydrolysis of the yeast cell wall is added to 1000 ml o...

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Abstract

The invention discloses a method for preparing water-insoluble beta-1, 3 / 1, 6-qlucan. The method has the steps as follow: 1)adding lysozyme into yeast cell wall suspending liquid for enzymolysis, centrifugation and collection at a temperature between 35 and 40 DEG C; 2) putting the product achieved from step 1) into NaOH solution and stirring for centrifugation and collection at a temperature between 35 and 4 DEG C; 3) depositing the product achieved from step 2) into acetic acid solution having concentration expressed in percentage by volume of 2-8 percent and stirring for centrifugation and collection; 4) stirring and washing the deposition after acid treatment and centrifugation of the step 3)by absolute ethyl alcohol and acetone and ether extract sequentially, and laying aside the treated ether extract for 12 to 24 hours, and drying in the centrifugation and collection way to achieve water-insoluble beta-1, 3 / 1, 6-qlucan. The invention is simple in control, and the beta-1,3-qlucan and the 1,6-qlucan prepared by the method have high purity and yield, and has the protein content and the fat content less than 1 percent.

Description

technical field [0001] The invention relates to a preparation method of water-insoluble β-1,3 / 1,6-glucan. Background technique [0002] Yeast is a single-celled fungus and is the most widely used and economically valuable microorganism so far. The international FDA recognizes brewer's yeast as a safe microorganism and has been widely used in the production of beverages and foods. At present, my country consumes about 2,000 tons of yeast annually and produces about 3 tons of waste yeast sludge. Since the yeast cell wall is the main by-product of the beer manufacturing industry, and the main treatment method for it today is to sell it cheaply as roughage or discharge it directly, it causes a lot of waste of resources and pollution to the environment. [0003] Several research results have shown that pure yeast cell walls have obvious immune-boosting effects on animals, and also exhibit growth-promoting effects. Further evidence shows that the main mechanism is that the β-1,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12P19/04
Inventor 呙于明张博
Owner CHINA AGRI UNIV
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