Method for producing validamycin by circulating fermentation

A technology of Jinggangmycin and its production method, which is applied in the field of circular fermentation to produce Jinggangmycin, can solve problems such as environmental pollution, and achieve the effects of reducing production costs and eliminating emissions

Inactive Publication Date: 2008-03-05
武汉天惠生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problem of environmental pollution caused by the bacterial residue and waste water produced by the fermentation of Jinggangmycin, the present invention provides a new method for the production of Jinggangmycin by circular fermentation

Method used

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  • Method for producing validamycin by circulating fermentation
  • Method for producing validamycin by circulating fermentation
  • Method for producing validamycin by circulating fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1 seed and fermentation culture

[0051] 1. Seed Preparation

[0052] 1.1 Strain: Streptomyces jinggang NFY-1022 strain

[0053] 1.2 Medium

[0054] 1.1.1. Slant medium (mother slant and eggplant bottle slant, each content percentage in the medium is weight percentage, the same below)

[0055] Glucose 1.0% Asparagine 0.05% KH 2 PO 4 0.05%

[0056] Agar 1.6% pH Natural

[0057] 1.1.2 Seed medium

[0058] Rice flour 4.0% Peanut meal powder 1.0% Yeast powder 0.5%

[0059] Soybean Meal Powder 1.0% Peptone Powder 0.5% KH 2 PO 4 0.025%

[0060] CaCO 3 0.3% NaCl 0.2% pH Natural

[0061] 1.3 The seed preparation process is shown in Figure 1:

[0062] 1.4 Preparation of slant medium

[0063] 1.4.1 Weighing and calculation: first calculate according to the proportional requirements, and then weigh them separately. The water used for preparing slopes should be distilled water or synthetic water, and agar powder or good agar strips shou...

Embodiment 2

[0166] Example 2 Jinggangmycin Circulating Fermentation Medium

[0167] Formula 1: 9% rice flour, 11% Jinggangmycin wet fungus residue (moisture content 75%), 0.15% soybean cake powder, 0.7% yeast powder; 3% calcium carbonate, pH7.2 (before disinfection).

[0168] Formula 2: 10% rice flour, 12% Jinggangmycin wet fungus residue (moisture content 76%), 0.15% soybean cake powder, yeast powder (0.7%, calcium carbonate 4%, pH 7.2 (before disinfection).

[0169] Formula 3: 9.5% rice flour, 13% Jinggangmycin wet fungus residue (moisture content 75%), 0.15% soybean cake powder, 0.7% yeast powder, 3% calcium carbonate, pH7.2 (before disinfection).

[0170] Formula 4: 10% rice flour, 14% Jinggangmycin wet fungus residue (moisture content 76%), 0.15% soybean cake powder, 0.7% yeast powder, 3% calcium carbonate, pH7.2 (before disinfection).

[0171] Formula 5: 5% corn starch, 30% Jinggangmycin wet fungus residue (moisture content 50%), 0.1% soybean cake powder, 5% peanut cake powder, 0.5...

Embodiment 3

[0174] Embodiment 3 60 liters fermenter fermentation

[0175] 1.1 Seed culture medium (each percentage in the culture medium is weight percentage, the same below) rice flour 5.0% peanut meal powder 0.5% yeast powder 0.5% KH 2 PO 4 0.025% CaCO 3 0.3% NaCl 0.2% PH natural

[0176] 1.2 Original fermentation medium (CK)

[0177] Rice flour 10% Soybean meal 2.5% Yeast powder 0.5%

[0178] K H 2 PO 4 0.30% CaCO 3 0.1% NaCl 0.15% pH7.0

[0179] 1.3 Using Example 2 Formula 1 as Jinggangmycin Wet Slag Fermentation Medium

[0180] 1.4 Process control

[0181] 1.4.1 After inoculation of the seed tank, the fermentation temperature is 40°C, and the stirring speed is 200rpm.

[0182] 1.4.2 The fermentation period of the fermenter is 41 hours, the inoculation amount is 5%, the fermentation temperature is 40°C, the tank pressure is 0.05Mpa, the ventilation rate: 0-8 hours 1: 1.2v / v / min; 8-41 hours 1: 1.5v / v / min, stirring speed 250rpm.

[0183] 1.4.3 The fungus residue filtere...

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Abstract

The present invention is circulating fermentation process for producing jinggangmycin. The circulating fermentation process has the fermented residue and effluent reused completely in the culture medium of the next time. The present invention has the advantages of no discharged waste slag and drained waste water.

Description

technical field [0001] The invention relates to a production method of biological pesticides, in particular to a new method for producing Jinggangmycin through fermentation. Background technique [0002] Jinggangmycin is a secondary metabolite produced by a strain of Streptomyces discovered by Chinese scientists in the soil of the Jinggangshan area in 1972. It is an agricultural antibiotic independently developed by my country. For more than 30 years, Jinggangmycin has made great contributions to the high and stable yield of rice in my country, and has become a well-known ideal biopesticide for Chinese farmers. [0003] Jinggangmycin is produced by the strain of Streptomyces hygroscopicus Jinggang, which is similar to validamycin A in Japan. Both are composed of 8 main components such as A, B, C, D, E, F, G, and H. Its most important component is Jinggangmycin A. Jinggangmycin A is a high-efficiency, low-toxic fungicide with long-lasting effect, rain-resistant, safe to use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/46C12R1/55
Inventor 朱世炎林开春毛光平张用梅李贤骆雪梅孙松柏邹大庆
Owner 武汉天惠生物工程有限公司
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