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Method for producing beta-carotene by solid-state fermentation of sitophila vein bacterium

A solid-state fermentation, carotene technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as difficulty in expanding the scale, inability to meet market demand, etc., and achieve short growth and reproduction cycles and abundant nutrients in bacteria. , the effect of improving productivity

Inactive Publication Date: 2008-03-05
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Several companies such as Natritite in the United States and Betatene in Australia produce natural β-carotene through breeding Dunaliella salina, with an annual production capacity of more than 30 tons. However, the cultivation of salina is strictly limited by external environmental conditions, making it difficult to expand the scale. , far from meeting the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: A method for producing β-carotene by solid-state fermentation using basic medium

[0028] Soybean dregs 90%, bran addition 10%, medium water content 77-79%, pH 6.0, sterilized at 121°C for 30 minutes.

[0029] After the culture medium is sterilized, it is cooled to 30℃, and then 6~9×10 6 Inoculation of spore bacteria suspension per ml, the inoculation amount is 9%. Place a constant temperature incubator for aerobic culture at 28-30°C for 96-120 hours. After the fermentation, the sporophytes were collected and broken by hot acid. After the broken walls, acetone organic solvent was used for multiple separation and extraction. The yield of β-carotene cells was 708.36 μg / g.

Embodiment 2

[0030] Example 2: A method for solid-state fermentation to produce β-carotene by adding a fermentation assistant—tomato juice to a basic medium

[0031] Add 2ml / 100ml tomato juice to 100g basal medium, 85% soybean dregs, 15% bran addition, and 77% water content of the medium. The addition amount is 7.5ml, the pH value is 6.0, and it is sterilized at 121°C for 30 minutes.

[0032] After the culture medium is sterilized, it is cooled to 30℃, and then 6~9×10 6 Inoculation of spore bacteria suspension per ml, the inoculation amount is 9%. Place a constant temperature incubator for aerobic culture at 28-30°C for 96-120 hours. After the fermentation, the sporophytes were collected and broken by hot acid. After the broken walls, acetone organic solvent was used for multiple separation and extraction. The yield of the carotene cells was 984.13 μg / g.

Embodiment 3

[0033] Example 3: A method for solid-state fermentation to produce β-carotene by adding a fermentation aid-orange peel juice to the basic medium

[0034] The basal medium 100g, the bean dregs 92%, the added amount of bran 8%, and the medium water content of 77% add 6g / 100ml orange peel juice, and the added amount is 6.25ml. pH 6.0, sterilize at 121°C for 30 minutes.

[0035] After the culture medium is sterilized, it is cooled to 30℃, using 6~9×10 6 Inoculation of spore bacteria suspension per ml, the inoculation amount is 9%. Place a constant temperature incubator for aerobic culture at 28-30°C for 96-120 hours. After the fermentation, the sporophytes were collected and broken by hot acid. After the broken walls, acetone organic solvent was used for multiple separation and extraction. The yield of carotene cells was 858.21ug / g.

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PUM

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Abstract

The present invention discloses solid N. sitophila fermenting process of producing beta-carotene. The process adopts N. sitophila strain No. 17 as the producing bacteria, culture medium compounded with soybean residue and wheat bran, and optional fermenting assistant(s) or fermenting promoter(s) in solid state fermentation with or without lighting for aerobic culturing. The fermented product is treated through breaking spore wall with hot hydrochloric acid, extracting with acetone extractant, rotary evaporation to eliminate organic solvent and chromatographic separation and purification in alumina or magnesia column to compound beta-carotene product. The present invention has the advantages of optimized bacterial strain, simple production process, no pollution, etc.

Description

Technical field [0001] The invention relates to a method for producing β-carotene by solid-state fermentation of Pseudomonas edulis. Background technique [0002] Beta-carotene is a category A excellent nutritional food additive recognized by the Joint Expert Committee of Food Additives of the United Nations Food and Agriculture Organization and the World Health Organization. The annual demand in the world is more than 1000t, but the current global output is only about 600t, which has great market development potential. . From an economic perspective, if the synthetic β-carotene is calculated at US$400 / kg, the world’s annual sales are estimated to reach US$200-250 million, and the prospects for economic development are also considerable. [0003] At present, there are three main production methods for natural β-carotene. The first is to directly extract from natural plants. For example, by extracting and separating from carrots and other vegetables containing trace β-carotene, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 吴耘红冮洁吴红艳刘晓兰郑喜群郭成宇杨志宏
Owner QIQIHAR UNIVERSITY
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