Preparation method for immobilization microorganism

A technology for immobilizing microorganisms and microorganisms, applied in the direction of being immobilized on/in an organic carrier, to achieve the effects of good stability, high mechanical strength and activity, good application prospects and practical value

Inactive Publication Date: 2008-04-23
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of preparation method of immobilized microorganism for the deficiencies of the existing method of solidifying cells with polyvinyl alcohol and sodium alginate, which has the ability to prepare immobilized microorganism with high activity, good mechanical properties and stability, Non-toxic, and solve the sticking problem during the preparation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The concrete method step of a kind of preparation method of immobilized microorganism is as follows:

[0022] (1) Preparation of microbial mixture

[0023] The mass ratio of 1.1% sodium alginate solution: 11% polyvinyl alcohol solution is 1:10, in a container, mix 1.1% sodium alginate solution and 11% polyvinyl alcohol solution and stir evenly , to prepare a carrier material solution. Then the solidified microorganisms are added to the carrier material solution to prepare a microorganism mixed solution. The mass ratio of microorganisms:carrier material is 1:5.

[0024] (2) Carry out the first step of solidification of microbial balls

[0025] After the first step (1) was completed, first in the calcium chloride solution with a mass concentration of 4%, the pH value was adjusted to 6.6 with sodium hydroxide solution, and then the microbial mixed solution prepared in the (1) step was added dropwise to the adjusted In the calcium chloride solution after the pH value, t...

Embodiment 2

[0031] The concrete method step of a kind of preparation method of immobilized microorganism is as follows:

[0032] (1) Preparation of microbial mixture

[0033] According to the mass ratio of 1.5% sodium alginate solution: 9% polyvinyl alcohol solution is 1:6, in a container, mix 1.5% sodium alginate solution and 9% polyvinyl alcohol solution and stir evenly , to prepare a carrier material solution. Then the solidified microorganisms are added to the carrier material solution to prepare a microorganism mixed solution. The mass ratio of microorganisms:carrier material is 1:4.

[0034] (2) Carry out the first step of solidification of microbial balls

[0035] After the first step (1) was completed, first in the calcium chloride solution with a mass concentration of 1%, the pH value was adjusted to 6.7 with sodium hydroxide solution, and then the microbial mixed solution prepared in the (1) step was added dropwise to the adjusted In the calcium chloride solution after the p...

Embodiment 3

[0041] The concrete method step of a kind of preparation method of immobilized microorganism is as follows:

[0042] (1) Preparation of microbial mixture

[0043] According to the mass ratio of 1% sodium alginate solution: 12% polyvinyl alcohol solution is 1:12, in a general container, mix 1-1% sodium alginate solution and 12% polyvinyl alcohol solution and stir evenly to prepare a carrier material solution. Then the solidified microorganisms are added to the carrier material solution to prepare a microorganism mixed solution. The mass ratio of microorganisms:carrier material is 1:6.

[0044] (2) Carry out the first step of solidification of microbial balls

[0045] After the first step (1) was completed, first in the calcium chloride solution with a mass concentration of 5%, the pH value was adjusted to 6.5 with sodium hydroxide solution, and then the microbial mixed solution prepared in the (1) step was added dropwise to the adjusted In the calcium chloride solution afte...

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Abstract

The present invention is process of preparing immobilized microbe and relates to microbe immobilizing method. The immobilizing process of the present invention adopts PVA and sodium alginate as the embedding material and sodium sulfate to replace boric acid, and includes a two-step immobilizing reaction with calcium chloride and sodium sulfate. The prepared immobilized microbe has no adhesion, less influence of the reaction reagents and reaction conditions on the activity of cell, no toxicity, simple operation, high strength and high stability. The preparation process may be performed continuously, and possesses excellent application foreground in biological waste water treatment, biomedicine preparation, food fermentation and other fields.

Description

technical field [0001] The invention belongs to the technical field of biological immobilization, in particular to a method for immobilizing microorganisms. Background technique [0002] After biocatalysts (such as enzymes, microorganisms, animal and plant cells, organelles, etc.) are immobilized by physical or chemical methods, their catalytic performance is improved and their use efficiency is greatly improved. Now the application research of immobilized biocatalyst has been involved in various fields such as food and fermentation industry, chemical synthesis industry, environmental pollution control and detection. [0003] Bio-immobilization technology is an emerging technology in the field of modern bioengineering, and its application scope involves many fields such as biology, organic chemistry, chemical analysis, medical diagnosis, environmental purification and energy production. The immobilization method and conditions have an important impact on the performance of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04
Inventor 霍丹群侯长军秦力杨丽敏
Owner CHONGQING UNIV
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