Lactococcus lactis food-sate secretion expression carrier and its preparing method and application

Lactococcus lactis, secreted expression technology, applied in the direction of bacterial antigen components, introduction of foreign genetic material using vectors, pharmaceutical formulations, etc., can solve the problems that expressed proteins cannot be folded correctly, exogenous protein delivery, and biological activity cannot be maintained
CN101168741AActive Publication Date: 2008-04-30ICDC CHINA CDC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ICDC CHINA CDC
Publication Date
2008-04-30

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Abstract

The invention relates to lactic acid galactococcus food-grade secretion expression vector, and the preparation method and the application thereof, in particular to the lactic acid galactococcus food-grade secretion expression vector which comprises the following components: a thyA gene selection marker, replicons of plasmid pWV01, multiple cloning sites, promoter used for secretion expression, a ribosome bind site of Usp45, a signal peptide sequence of Usp45 and a partial Usp45 mature peptides coded sequence.
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Description

technical field

[0001] The invention relates to the field of bioengineering, in particular to a secreted expression carrier capable of secreting and expressing foreign genes in Lactococcus lactis and its preparation method and application. Background technique

[0002] Food-grade expression systems are gaining increasing attention due to their safety and compliance with FDA requirements. The food-grade expression system must meet the following conditions: (1) The vector must be food-grade and must not contain non-food-grade functional DNA fragments, such as antibiotic resistance genes, etc.; (2) The expression host must be safe, with clear and stable characteristics Food-grade microorganisms (generally regarded as safe, GRAS), such as Lactococcus lactis, Lactobacillus acidophilus, Bifidobacterium and other strains that have been widely used in the food industry for a long time. In addition, the host bacteria of a food-grade system must be sufficiently stable in food and aft...

Claims

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