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Method of determining iron concentration

A determination method, iron chelate technology, applied in the direction of chemical method analysis, biological test, measuring device, etc., to achieve the effect of accurate determination

Inactive Publication Date: 2008-04-30
WAKO PURE CHEMICAL INDUSTRIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the problem with the above method is that the measured value obtained by the direct method using the automatic analyzer is compared with the measured value obtained by the international standard method, and the measured value of the direct method is lower than the measured value of the international standard method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0061] According to the international standard method, the standard addition method of the international standard method (in the international standard method, the liquid volume is reduced due to the removal of protein, so in order to correct it, 3 equivalents (concentration) of iron are added to the sample for measurement, according to its regression The intersection point of the X-axis of the formula to obtain the iron concentration in the sample) and the existing direct method using an automatic analyzer to measure the iron concentration in the serum of the same serum samples 1 to 5, and compare the respective measured values.

[0062] (1) International Standard Law

[0063] According to the method described in the summary of the clinical examination method (revised 32nd edition, first printing, 580 pages, edited by Masamitsu Kanai, published on February 20, 2017, Kanehara Publishing Co., Ltd.), the procedure was performed as follows.

[0064] [Preparation of reagents]

[...

Embodiment 1

[0125] [Preparation of test solution]

[0126] (R-1) buffer

[0127] 400mM glycine buffer (pH3.5) containing 10mM lithium chloride, sodium chloride, potassium chloride or cesium chloride (10mM for alkali metal ions), 40mM ascorbic acid, and surfactant

[0128] (R-2) Chromogenic test solution

[0129] 40mM glycine buffer (pH3.0) containing 1.86mM sodium phenanthroline sulfonate and surfactant

[0130] [Measurement of Serum Iron Concentration]

[0131] Serum samples 6 to 10 were measured using the above-mentioned test solution with a Hitachi 7170S automatic analyzer.

[0132] 〔Measurement conditions〕

[0133] The measurement parameters were set as follows, and the serum iron concentration in each sample was measured.

[0134] Determination method: 2-point endpoint method[16]-[34]

[0135] Sample volume: 12μL

[0136] R-1: 160 μL

[0137] R-2: 40 μL

[0138] Measurement wavelength: 600 / 546nm

[0139] Measuring temperature: 37°C

[0140] Standard concentration: 200μg / dL...

Embodiment 2

[0152] In addition to adding lithium chloride to the "(R-1) buffer solution" to a concentration of 0.1mM, 1mM, 10mM or 100mM (lithium ion concentration at the time of measurement is 0.075mM, 0.75mM, 7.5mM, 75mM, respectively) , using the reagents prepared in the same manner as in Example 1, the iron concentrations of serum samples 11 to 15 were measured in the same manner as in Example 1.

[0153] The results are shown in Table 3.

[0154] As a control, using the same serum sample, it was measured by the standard addition method of the international standard method of Reference Example 1. The results are shown in Table 3 together.

[0155]In Table 3, the ratio of the measured values ​​is shown in the () below each average value. In the ratio of the measured values, the measured value obtained by measuring the standard addition method based on the international standard method is 100, and calculated in each The proportion of the measured value obtained by the direct method in...

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Abstract

A method of determining the concentration of iron in a sample, characterized by bringing the iron contained in the sample into contact with an iron-chelating color former in the presence of lithium ions and determining the iron concentration based on the degree of the resultant coloring; and a reagent and kit for iron determination which are for use in the method. This method eliminates the conventional problem that found values obtained are low as compared with ones obtained by the international standard method. This method does not necessitate a complicated operation, e.g., protein removal, and enables the determination of an iron concentration with an autoanalyzer. Since found values obtained by the method are close to ones obtained by the standard addition method according to the international standard method, more accurate determination is possible.

Description

technical field [0001] The invention relates to a method for measuring the iron concentration in a sample by a direct method using an iron chelate chromogenic agent. In the method, the determination is carried out under the coexistence of lithium ions. Background technique [0002] Measurement of iron concentration in serum and plasma is used for diagnosis of anemia, iron deficiency anemia, liver cirrhosis, etc., and is one of important measurement items in the field of clinical examination. [0003] As a method of measuring the iron concentration, for example, a colorimetric analysis method using various metal chelate color reagents such as bipyridine and o-phenanthroline is generally used. When sensitivity, specificity, solubility, etc. are considered, bathophenanthroline, 2-nitroso-5-(N-propyl-N-sulfopropylamino)-phenol (nitroso PSAP), 3 -(2-pyridyl)-5,6-bis[2-(5-furanylsulfonic acid)]1,2,4-triazine disodium salt, tripyridyl triazine, iron zinc body and other iron chelat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/77G01N31/00G01N31/22
CPCG01N33/52G01N21/80G01N21/78G01N31/22G01N33/84
Inventor 赤羽和井尻晴久花田寿郎
Owner WAKO PURE CHEMICAL INDUSTRIES
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