Method for establishing brazilian panama rubber tree suspending cell line

A technology of Hevea brasiliensis and suspension cells, which is applied in the field of establishment of Hevea brasiliensis suspension cell lines, can solve problems such as the inability to obtain a large number of autogenous clone plants, the inability to obtain embryogenic callus at low cost, and the inability to proliferate embryogenic callus. Achieve the effect of low production cost, wide application field and high efficiency

Inactive Publication Date: 2008-05-07
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

However, the main problems in the production of self-rooted clonal plants of Hevea brasiliensis are: the acquisition of anthers and inner integuments is limited by seasons, it is impossible to obtain embryogenic callus in a cheap, large, continuous and long-term manner, and it is impossible to control embryogenic callus. Large-scale multiplication of sexual callus, so it is impossible to obtain a large number of self-rooted clones, and thus unable to meet the demand for a large number of self-rooted clones in field production

Method used

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Embodiment

[0024] A method for establishing a suspension cell line of Hevea brasiliensis, the specific steps are:

[0025] A. Taking the buds of Hevea brasiliensis as the explants, the buds were surface-sterilized with 75% alcohol for 60 seconds, then sterilized with 0.1% mercuric chloride for 15 minutes, and finally rinsed with sterile water for 3 times, 5 minutes each time;

[0026] B. Peel off the stamens in the sterilized flower buds, inoculate them on the induction medium and carry out dark culture for 50 days at a temperature of 28°C; the induction medium is based on MS medium, and MS is basically cultured The content of potassium dihydrogen phosphate, anhydrous calcium chloride, manganese sulfate tetrahydrate and magnesium sulfate heptahydrate in the base was adjusted to potassium dihydrogen phosphate 350mg / L, anhydrous calcium chloride 200mg / L, manganese sulfate tetrahydrate 20mg / L L, magnesium sulfate heptahydrate 400mg / L, and add 2,4-D 2mg / L, KT 1mg / L, NAA 1mg / L, Phytagel 2g / L,...

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Abstract

The invention discloses a method building a suspension cell line of hevea brasiliensis, which comprises a preparing process of explant, an induction process of embryogenic callus, a continuing culture process of embryogenic callus, and a suspended culture process of embryogenic callus. The invention has the advantages of simple technological process, low production cost, rapid and steady proliferation and long term continuing culture of the built suspension cell line; solving the problem of the influence to the obtaining of the general hevea brasiliensis embryogenic callus from the season and climate, scale proliferation, and the labour and material resource consumption; providing an important foundation for the large-scale commercialization, factory production and large-scale field planting of the hevea brasiliensis self-rooted clone; providing new way and condition for creating new excellent germplasm resources of hevea brasiliensis utilizing the biotechnology methods of somatic hybridization, genetic transformation and mutant induction; laying a foundation for the research and development of hevea brasiliensis bioreactor.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and more particularly relates to a method for establishing a suspension cell line of Hevea brasiliensis. Background technique [0002] Natural rubber is one of the four major industrial raw materials and an extremely important strategic material. At present, among about 2000 kinds of rubber-producing plants in nature, about 99% of commercial natural rubber comes from Hevea brasiliensis. Hevea brasiliensis is a perennial tree belonging to the family Euphorbiaceae. The conventional breeding cycle is 20 to 30 years. The long breeding cycle limits the selection and renewal of new varieties of rubber tree in Brazil. In order to broaden the breeding methods and innovative breeding methods of Brazilian rubber tree, since the 1950s, breeders from all over the world have begun to study the application of biotechnology in the breeding of Brazilian rubber tree. The success of somatic embryo...

Claims

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Application Information

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IPC IPC(8): C12N5/02C12N5/04
Inventor 华玉伟黄华孙黄天带吴紫云曾霞胡彦师李维国李哲
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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