Diesel oil alkane component degradation bacterium and application thereof

A technology of alkane degrading bacteria and degrading bacteria, applied in the direction of bacteria, water pollutants, water/sludge/sewage treatment, etc., to achieve good adaptability, stable and efficient degradation performance, and efficient alkane degradation ability

Inactive Publication Date: 2008-05-28
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a bacterial strain that can degrade petroleum substances effi

Method used

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  • Diesel oil alkane component degradation bacterium and application thereof

Examples

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Effect test

Embodiment 1

[0013] Example 1: Screening and isolation of alkane-degrading bacteria Burkholderia cepacia GS3C and its alkane-degrading performance

[0014] Collect the sludge mixture from the sewage treatment station of Guangzhou Petrochemical General Plant, take 5g of soil samples in 100ml of sodium pyrophosphate solution, place them in a shaker and shake overnight. With n-hexadecane as the sole carbon source, the shaking liquid was inoculated into inorganic salt medium, and acclimatized in a shaker at 30C and 150 rpm. The composition of inorganic salt medium (MSN) is as follows (g / L): MgSO 4 ·7H 2 O, 0.2; CaCl 2 2H 2 O, 0.02; K 2 HPO 4 , 0.5; KH 2 PO 4 , 0.5; NH 4 NO 3 , 0.5; trace element solution, 10ml / L. The composition of the trace element solution is (mg / L): FeSO 4 ·7H 2 O, 300; MnSO 4 ·H 2 O, 50; CoCl 2 ·6H 2 O, 106; Na 2 MoO 4 2H 2 O, 34; ZnSO 4 ·7H 2 O, 40; CuSO 4 ·5H 2 O, 50. Adjust the pH to 7.0.

[0015] A strain of alkane-degrading bacteria GS3C was o...

Embodiment 2

[0018] Example 2: The effect of the addition of yeast extract on the degradation of different initial concentrations of carbon sources by alkane-degrading bacteria Burkholderia cepacia GS3C

[0019] Using n-hexadecane as the carbon source, the alkane-degrading bacteria Burkholderia cepacia GS3C was inoculated into the inorganic salt medium and cultured for 4 days. A control group was set up without and with yeast extract solution (600mg / L), and the concentration of the carbon source was determined respectively. The degradation rate and degradation rate of n-hexadecane by degrading bacteria at 750, 3750, and 7500 mg / L. It can be seen from Table 1 that the degrading bacteria GS3C can still grow normally under the high substrate concentration of 7500mg / L. The available carbon source and energy are sufficient, but the degradation rate keeps increasing. At the same time, the addition of yeast extract can significantly promote the degradation of alkane-degrading bacteria Burkholder...

Embodiment 3

[0024] Example 3: Effect of heavy metal Cd on the degradation of alkane-degrading bacteria Burkholderia cepacia GS3C

[0025] Inoculate the alkane-degrading bacteria Burkholderia cepacia GS3C into the inorganic salt medium containing n-hexadecane at a concentration of 750 mg / L, and add Cd at a concentration of 0.3, 3, and 9 mg / L, respectively 2+ , while without adding Cd 2+ The sample was used as a control, and the degradation rate of n-hexadecane was measured after 4 days. As can be seen from Table 2, in the experimental concentration range (0-9mg / L) Cd 2+ It has no toxic effect on degrading bacteria, but has a certain promoting effect.

[0026] This embodiment illustrates that the higher Cd of environmental biological toxicity 2+ It is not only non-toxic to alkane-degrading bacteria Burkholderiacepacia GS3C, but also has a certain promoting effect, and has the potential to be applied to the remediation of petroleum-heavy metal (Cd) complex pollution systems.

[0027] Eff...

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Abstract

The invention discloses to a strain of degrading bacteria for alkenes components of diesel and usage thereof. The strain of degrading bacteria for alkenes components of diesel of the invention is Burkholderia cepacia which is capable of degrading n-hexadecane of 750mg/L to be of 200mg/L within four days, and cadmium (Cd) with low concentration (<9mg/L) accelerates the degradation to some extent. When the diesel is taken as the unique carbon source, the degrading bacteria can better degrade the alkenes components (C12-C30) of the diesel. Compared with other bacterial strains, the strain of the invention has the advantages of high effective capability of degrading alkenes, perfect adaptability and tolerant property of Cd, which can be well applied in the field of bioremediation for water body which is polluted by oil pollutants and polluted by the combination of both oil and heavy metals (Cd).

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a bacterium for degrading diesel alkane components and its application in wastewater biological treatment and environmental pollution restoration. Background technique [0002] Petroleum and its products are widely used in the world at present, and the problems of water and soil pollution caused by them are becoming more and more serious, and people are paying more and more attention to them. The total oil production in the world today is about 2.2 billion tons per year, of which 1.75 billion tons are produced by land oil fields. Oil pollution alone has 8 million tons entering the environment every year in the world, and nearly 600,000 tons enter the environment every year in my country, polluting soil, groundwater, rivers and oceans. After these petroleum substances enter the soil environment, a series of physical and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/32
Inventor 党志吴仁人
Owner SOUTH CHINA UNIV OF TECH
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