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Sterilizing method of trypsin inhibitor

A technology of trypsin inhibition and sterilization method, which is applied in the field of sterilization of trypsin inhibitor products, can solve the problems of difficult membrane cleaning, small processing capacity, expensive equipment, etc., achieve good sterilization effect, large processing capacity, and production low cost effect

Inactive Publication Date: 2010-10-13
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, if the method of high temperature sterilization is adopted, the activity of trypsin inhibitor products will be inactivated; and the use of membrane filtration sterilization technology has problems such as expensive equipment, small processing capacity and difficult cleaning of the membrane.

Method used

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  • Sterilizing method of trypsin inhibitor
  • Sterilizing method of trypsin inhibitor
  • Sterilizing method of trypsin inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Pressure sterilization of trypsin inhibitor and its microbial detection

[0040] Several different protein powders were made into a 50 mg / ml solution, centrifuged at 1000 × g for 30 min at 5°C, and the supernatant was divided into two parts, one for the determination of trypsin activity and microbial indicators. The other part was pressurized for 60 min under the conditions of 600MPa and 25°C, and the activity of trypsin inhibitor and microbial indicators were determined. Among them, the total number of colonies is carried out with reference to the GB / T 4789.2-2003 detection method; the total number of coliforms is carried out with reference to the GB / T 4789.3-2003 testing method; the mold and yeast are carried out with reference to the GB / T 4789.1 5-2003 testing method. The test results are shown in Table 1.

[0041] In Table 1, sweet potato crude protein is the sweet potato crude protein prepared by the above method, wherein the protein content is about 70...

Embodiment 2

[0045] Example 2. Effect of trypsin inhibitor concentration on its activity during sterilization

[0046] The purified sweet potato protein Sporamin was diluted into 3, 2, 1, 0.6, 0.3, 0.2, 0.15, 0.1 mg / ml solutions with different concentrations, and kept at 600 MPa and 25 °C for 20 min. active.

[0047] figure 2 That is, the relationship between the concentration of Sporamin and the percentage of trypsin inhibition. It can be seen from the figure that the Sporamin concentration has different effects on the trypsin inhibitor activity. At 0.1mg / ml, the inhibition rate of Sporamin to trypsin (80μg / ml) can reach 50%; when the concentration of Sporamin is between 0.1-0.3mg / ml, increasing the concentration of Sporamin can significantly inhibit trypsin. Increase; at 0.6mg / ml its inhibition rate can reach 90%. But between 0.6-3mg / ml concentration, the inhibition rate of Sporamin to trypsin did not change significantly.

[0048] Using the exact same experimental conditions as Sp...

Embodiment 3

[0049] Example 3. Effect of sterilization pressure on the activity of trypsin inhibitor

[0050] Under the condition of temperature of 25℃, under the pressure of 200MPa, 300MPa, 400MPa, 500MPa and 600MPa, the sweet potato protein Sporamin trypsin inhibitor solution with a concentration of 0.6mg / ml was pressurized for 20min. Inhibitory activity.

[0051] Using the exact same experimental conditions as Sporamin, the activity of soybean trypsin inhibitor was determined.

[0052] image 3 That is, the relationship between the effects of different pressures on the activities of sweet potato protein Sporamin and soybean protein trypsin inhibitor. Depend on image 3 It can be seen that under the pressure condition of 200-600MPa for 20min, there is no significant effect on the trypsin inhibitor activity of Sporamin and soybean.

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Abstract

The invention discloses a sterilization method of trypsin inhibitor products, which is to put trypsin inhibitor in the conditions of 200-800 MPa for sterilizing, wherein, the sterilization pressure preferentially chooses 200-600 MPa and more preferentially chooses 600 MPa, the sterilization temperature is 25-50 DEG C, and the sterilization time is 5-60 minutes and preferentially chooses 20-60 minutes. The sterilization method provided by the invention can not only keep the activity of trypsin inhibitor in a sample solution from substantially changing, but also can play a good sterilizing rolefor leading microorganism content in the sample solution to accord with health food universal sanitary requirements, wherein, the total number of bacterial colony and the detecting results of coliform group bacteria, mould, saccharomycete reach the sanitary requirements, and no pathogens are detected. The method has extensive application prospects in food processing field and high economic value.

Description

technical field [0001] The present invention relates to a sterilization method for trypsin inhibitor products, in particular to a method utilizing ultra-high pressure sterilization. Background technique [0002] Trypsin inhibitor (TI) commonly exists in animals, plants and microorganisms, such as aprotinin (aprotinin), human urine trypsin inhibitor (UTI), soybean trypsin inhibitor (soybean trypsin) inhibitor, SBTI), pumpkin trypsin inhibitor (Cucurbitamaxima trypsin inhibitor, CMTI), etc. [0003] At present, most of the developed TIs are derived from humans and animals, such as human urinary trypsin inhibitor, aprotinin, etc. However, due to their large molecular weight and complex structure, it is difficult to extract, isolate, research, synthesize and further develop them. The therapeutic effect also has many disadvantages, such as being susceptible to contamination by pathogenic microorganisms such as viruses, and can cause allergic reactions. In order to overcome the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/02
Inventor 木泰华李郁
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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