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Use of aloperine in preparing medicament for treating chronic hepatitis B

A technology of chronic hepatitis B and aloperine, applied in the direction of drug combination, pharmaceutical formula, organic active ingredients, etc., can solve the problem that there is no anti-HBV effect of aloperine

Inactive Publication Date: 2008-07-02
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report about the anti-HBV effect of aloperine

Method used

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  • Use of aloperine in preparing medicament for treating chronic hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Anti-HBV research experiment of aloperine in vitro

[0018] 1. Drugs: Aloperine, matrine, and oxymatrine are provided by Jindou Plant Biochemistry Co., Ltd., Yanchi County, Ningxia Autonomous Region, with a content of more than 98%. Raw materials for lamivudine are provided by Shanghai Desano Company , the content is above 98%.

[0019] 2. Reagents and main instruments

[0020]DMEM medium is a product of Gibco, USA. Polyethylene glycol (PEG, AR), dimethyl sulfoxide (DMSO, AR), and calf serum (FBS, BR) are domestic reagents. MTT (thiazolyl blue, AR), hepatitis B virus s antigen detection kit (batch number 20040929) and hepatitis B virus e antigen detection kit (batch number 20041124) were purchased from Shanghai Industrial Kehua Biotechnology Co., Ltd.; hepatitis B virus pre-s1 antigen detection Kits were purchased from Shanghai Alpha Biotechnology Co., Ltd. (batch number 20050104); HBV DNA detection kits were purchased from Shenzhen Piji Biotechnology Co., ...

Embodiment 2

[0064] Example 2 Effect Test on Protein Tyrosine Kinase

[0065] 1) Medicine: with above-mentioned embodiment 1. .

[0066] 2) Reagents and main instruments

[0067] The DMEM medium is the product of Gibco Company in the United States, and the calf serum (FBS, BR) is a domestic reagent. Tyrosine Kinase Assay Kit was purchased from CHEMICONR International, Inc.

[0068] 3) cells

[0069] HepG2.2.15 cells are a cell line established by HBV DNA transfection of human liver cancer cell line HepG2, which can stably express viral proteins and secrete virus particles, etc. ), the medium is DMEM, containing 100ML / L fetal bovine serum, G418 200mg / L, 1×105U / L penicillin and streptomycin.

[0070] 4) Method: detection of protein tyrosine kinase activity

[0071] To prepare protein tyrosine kinase samples:

[0072] Cell culture: According to the conventional method, the cells were inoculated into 25cm2 culture flasks, each flask was a group, and a cell control group was set up. Cul...

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Abstract

The invention pertains to the pharmaceutical field of traditional Chinese medicine, and relates to an alkaloid monomer with anti-HBV, in particular to the usage of aloperine in the preparation of drugs for the treatment of chronic hepatitis B drug. The aloperine pertains to difused piperidine alkaloids, which is one of the matrine alkaloids, and the alkaloid monomer is one of the main effective ingredients of northwest special drug sophora alopecuroides. The results of HepG2.2.15 cell experiments show that, the aloperine has good anti-HBV function in vitro and good inhibition function particularly on the hepatitis B virus protein, the inhibition function on the hepatitis B virus protein is better than the similar matrine and oxymatrine, as well as lamivudine. The aloperine can be linked with the drugs which can inhibit the copy of HBV DNA, so as to improve the anti-HBV efficacy or further prepare the drugs for the treatment of chronic hepatitis B.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to alkaloid monomer isolated from leguminous plants - aloperine, and its application in the preparation of pharmaceutical compositions. Background technique [0002] my country is a high-incidence area for hepatitis B virus (HBV) infection. According to relevant survey reports, the adult HBV infection rate is 42.4%-80.77%, and the HBV carrier rate is 10.09%. Among them, 25%-40% of the causes of death are liver cirrhosis or liver cancer. . Another statistics show that the number of deaths due to hepatitis B-related diseases is as high as more than 1 million every year, and 15%-20% of chronic hepatitis B patients with active virus replication will develop liver cirrhosis within 5 years, and patients with chronic liver cirrhosis will develop liver cirrhosis within 5 years. The survival rate is only 55%. What is more noteworthy is that the risk of liver cancer in patie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4748A61P1/16A61P31/20
Inventor 陈建杰聂红明高月求金树根
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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