Technique for preparing oligomeric chitosan by complex enzyme degradation

A technology of chitosan oligosaccharides and process methods, which is applied in the field of chitosan oligosaccharides prepared by enzymatic methods, can solve the problems that the average molecular weight of degradation products is difficult to reach 1500 Da, the non-specific enzyme activity is limited, and the degradation time is long. Achieve the effect of improving the production environment, reducing size and operating costs, and high product activity

Inactive Publication Date: 2008-07-09
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no industrialized production of specific enzymes with better chitosan degradation performance, and various non-specific enzymes have limited activity, long degradation time, and the average molecular weight of degradation products is difficult to reach about 1500Da, and the distribution is wide

Method used

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  • Technique for preparing oligomeric chitosan by complex enzyme degradation
  • Technique for preparing oligomeric chitosan by complex enzyme degradation

Examples

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Effect test

Embodiment 1

[0029] Mix 200g of chitosan with a deacetylation degree greater than 95% (molecular weight is about 1 million Da to 600,000 Da) in 10L of water under stirring, add glacial acetic acid with a concentration of >99.5% to dissolve chitosan and adjust the solution The pH value is 4.5, the temperature is raised to 50°C, 12g of α-amylase is added, and 12g of bromelain is added after constant temperature reaction for 0.5h, and the constant temperature reaction is continued for 3.5h, and the discharge is cooled to room temperature; the obtained chitosan degradation solution is 10L, Its pH value is 4.7, add deionized water to dilute to 20L, and then use hollow fiber components for microfiltration; the operating conditions of microfiltration are: operating pressure difference 0.06-0.08MPa, operating temperature is room temperature, and every 0.5h in the microfiltration process Add 5L of deionized water to the feed liquid, and the total amount of added water is 10L; the microfiltration per...

Embodiment 2

[0031] Mix 200g of chitosan with a deacetylation degree greater than 95% (molecular weight is about 1 million Da to 600,000 Da) in 10L of water under stirring, add glacial acetic acid with a concentration of >99.5% to dissolve chitosan and adjust the solution The pH value is 4.6, warm up to 35°C, add 12g of α-amylase, react at a constant temperature for 0.5h, then add 12g of cellulase, continue the reaction at a constant temperature for 3.5h, and cool the material to room temperature; the obtained chitosan degradation solution is 10L , whose pH value is 4.8, dilute to 20L with deionized water, and then use hollow fiber components for microfiltration; the operating conditions of microfiltration are: operating pressure difference 0.06-0.08MPa, operating temperature is room temperature, and every 0.5 h Add 5L of deionized water to the feed liquid, and the total amount of added water is 10L; the microfiltration permeate is heated to 30°C and the concentrated volume of nanofiltratio...

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Abstract

The invention discloses a process for preparing low molecular weight chitosan through degrading compound enzyme. The synergistic effect which is produced in the process of degrading chitosan by various non-specific enzymes of alpha-amylase and bromelain or cellulose is utilized to increase the degrading efficiency, which enables the average molecular weight of products to degrade and reach about 1500 Da in 5.0 hours. After the final degradation products are microfiltration and nanofiltration purified, concentrated, and spray dried, the low molecular weight chitosan product with high quality can be obtained. Compared with a traditional process for preparation, the process not only has high product activity, good quality and convenient operation but also improves the manufacturing environment, which is beneficial to realize industrialization.

Description

technical field [0001] The invention belongs to a process for preparing chitosan oligosaccharide by enzymatic method. Background technique [0002] Chitosan is the product of partial deacetylation of chitin. It is a natural alkaline polysaccharide with a molecular weight ranging from hundreds of thousands to millions. The structure is as follows. [0003] [0004] Due to its large molecular weight, it is only soluble in dilute acid solution but not in water and alkaline solution, so its application is greatly limited. Chitosan oligomeric chitosan with a molecular weight lower than 1500 after degradation not only retains the high molecular weight lowering blood fat and cholesterol, enhances human immunity, inhibits the growth and metastasis of tumor cells, and has excellent hygroscopicity, moisture retention, and antibacterial properties. Due to its enhanced solubility, it is easy to be absorbed and utilized, and further exhibits many unique physiological activities and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12N9/26C12N9/42C12N9/50
Inventor 林强韩永萍
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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