Application of acetophenetidin in preparation of medicaments for testing liver cancer high risk group
A technology for phenacetin and liver cancer, a new use of phenacetin, and the application field of phenacetin in the preparation of drugs for detecting high-risk groups of liver cancer
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Embodiment 1
[0016] Embodiment 1: the preparation of phenacetin capsule
[0017] According to the relevant provisions of "Pharmacopoeia of the People's Republic of China" 2005 edition capsules, phenacetin (medicinal raw materials provided by Jiamusi Luling Pharmaceutical Co., Ltd.) is prepared into capsules for men (0.25 g / grain) and capsules for women (0.2125 g / capsule) Two specifications, 4 capsules per plate, packed in aluminum foil.
Embodiment 2
[0018] Embodiment 2: the preparation of phenacetin injection
[0019] According to the relevant regulations of "Pharmacopoeia of the People's Republic of China" 2005 edition injection, phenacetin (medicinal raw material is provided by Jiamusi Luling Pharmaceutical Co., Ltd.) is prepared into a 0.15% injection, and its solvent is ethanol: water for injection=15:85 (v / v), in aliquots of 2 ml medicinal glass vials. After sealing, autoclave.
Embodiment 3
[0020] Example 3: Research on phenacetin as a diagnostic drug for detecting high-risk groups of liver cancer
[0021] Objective: To study whether phenacetin, as a diagnostic drug, can be used to detect high-risk groups of liver cancer.
[0022] Instrument: 20A high performance liquid chromatography (manufactured by Shimadzu Corporation, Japan, including LC-20AD pump, DGU-20A online degasser, SIL-HTC autosampler, ODS c18 Chromatographic column 4.6×250 mm, mobile phase: acetonitrile-0.1% formic acid (30:70), isocratic elution, flow rate 1 mL / min, injection volume 20 μL).
[0023] Subjects of parallel experimental design: 24 patients with chronic hepatitis B, Child-Pugh grade A. Aged 26-59 years old, including 18 males and 6 females. Eighteen patients with primary hepatocellular carcinoma had a history of chronic hepatitis B, and the Child-Pugh classification was A. Aged 38-62 years old, including 13 males and 5 females. The normal control group consisted of 12 people, half m...
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