Purification method for tryptophane

A purification method and tryptophan technology, applied in the direction of organic chemistry and the like, can solve the problems of poor industrial applicability, high post-processing requirements, complicated and cumbersome processes, etc., and achieve the effects of convenient preparation, low cost, and simple process control.

Active Publication Date: 2008-08-20
汕头市佳禾生物科技有限公司
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  • Abstract
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Problems solved by technology

[0008] There are few domestic reports on tryptophan purification technology. "Research on Ion Exchange Adsorption of L-Tryptophan" (Acta Bioengineering, 1996, 12(4): 499-502) studies the adsorption of cation-exchange resin in aqueous solution. , elution to separate and purify L-tryptophan; "Separation of Tryptophan in Enzymatic Synthesis" (Industrial Microbiology, 1996, 26 (1): 27-30 pages) the tryptophan solution was first passed thr...

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  • Purification method for tryptophane

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Soak activated carbon in concentrated nitric acid, stir for 30 minutes, filter, wash with pure water until the washing liquid has no acid ions, dry, and then dry and activate at 120°C to obtain surface oxidized activated carbon.

[0022] 1.8 kg of tryptophan crude product with a content of 97.3wt% and a light transmittance of 53% was dissolved in 150 liters of pure water at 45° C., and 18 g of the above-mentioned surface-oxidized activated carbon was added and stirred for 30 minutes. The solution was passed through a 12 μm filter to remove the surface-oxidized activated carbon, and then used 0.22μm filter membrane for membrane filtration, take 50 liters of filtrate and concentrate it to about 17.5 liters in a pressure environment of 45°C and 10Kpa, cool to 15°C, stir for 1 hour, then heat up to 45°C to concentrate and continuously replenish the filtrate to maintain the volume of the concentrate About 23 liters, when the filtrate reaches 50 liters, cool down to 25°C, stir...

Embodiment 2

[0024] 1.8 kg of tryptophan crude product with a content of 97.3 wt% and a light transmittance of 53% was dissolved in 150 liters of purified water at 45°C, and 18 g of surface-oxidized activated carbon described in Example 1 was added, stirred for 30 minutes, and the solution was passed through a 12 μm filter to remove surface oxidation Activated carbon, and then use a 0.22μm filter membrane for membrane filtration, take 50 liters of filtrate and concentrate it to about 12 liters in a pressure environment of 60°C and 30KPa, cool to 25°C, stir for 2 hours, continue to heat up to 60°C to concentrate and continuously replenish the filtrate, Keep the concentrated volume at about 20 liters, add 50 liters of filtrate, cool down to 25°C, stir for 30 minutes, then raise the temperature to 60°C to concentrate and replenish the filtrate, keep the concentrated volume at about 20 liters, after adding all the filtrate, concentrate to 10 liter, cooled to 4°C, stirred for 2 hours, filtered, ...

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Abstract

The invention discloses a purification method of tryptophan; wherein, the tryptophan to be purified is dissolved in water; filtrate which is absorbed after being added with surface oxidative activated carbon is treated with vacuum condensation into a volumn which is 6 to 10 percent of the original volume at the temperature of 40 to 65 DEG C, and the temperature is reduced to 3 to 5 DEG C to carry out stirring and crystallization to obtain pure products; an intermittent concentration cooling crystallization mode is preferably chosen in the concentration process. The purification method basically has no three wastes; the mother liquor can be recycled and used for a plurality of times; the purification method is low in cost, simple in technical control, high in the purity of the obtained products and low in impurity content; and the purification method more conveniently obtains the tryptophan products which meet the requirements of the version 2005 of Chinese pharmacopoeia and the 29th version of American pharmacopoeia.

Description

technical field [0001] The invention relates to a method for purifying amino acid, in particular to a method for purifying tryptophan. Background technique [0002] Molecular Formula C of L-Tryptophan 11 h 12 N 2 o 2 , molecular weight 204.23, CAS No.73-22-3, the structural formula is as follows: [0003] [0004] L-tryptophan is one of the eight essential amino acids in the human body. In the living body, tryptophan can be converted into important physiologically active substances such as 5-hydroxytryptamine, indoleacetic acid, niacin, alkaloids and coenzymes, and participate in the regulation of protein synthesis. , plays an important role in the growth, development and metabolism of humans and animals, so it is of great social and economic significance to study and produce high-purity tryptophan products that are suitable for food and medicine. [0005] At present, the purification technology of tryptophan mainly adopts adsorption and ion exchange purification, ne...

Claims

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Application Information

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IPC IPC(8): C07D209/20
Inventor 章小亮黄云生袁爱国张楚扬陈德苞陈家荣
Owner 汕头市佳禾生物科技有限公司
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