Calciparine/sodium salt nano oral preparation and preparation technique thereof

The technology of oral preparation and heparin calcium is applied in the field of heparin calcium/sodium salt nano oral preparation and its preparation technology. The effect of good intestinal absorption rate, good stability and long metabolism time

Inactive Publication Date: 2008-08-27
褚红女
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low encapsulation efficiency of the low molecular weight heparin nano-liposomes prepared by the above method, it cannot reach the 80% standard stipulated in the "Chinese Pharmacopo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Calciparine/sodium salt nano oral preparation and preparation technique thereof
  • Calciparine/sodium salt nano oral preparation and preparation technique thereof
  • Calciparine/sodium salt nano oral preparation and preparation technique thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Preparation of low molecular weight heparin calcium nanoliposomes

[0029] Chitosan 1g is dissolved in the acetic acid solution 50mL of 0.1% concentration (w / w), obtains chitosan solution, filters, gets filtrate, and in the filtrate drips concentration is 1% (w / w) under magnetic stirring. Low-molecular-weight heparin calcium (Changzhou Tianpu Pharmaceutical Co., Ltd., batch number H1999007) aqueous solution until a milky white precipitate appears, the resulting suspension is centrifuged, and the precipitate is freeze-dried to obtain low-molecular-weight heparin calcium nanoliposomes. The encapsulation efficiency is as high as 98% (97.94±1.33%).

Embodiment 2

[0030] Embodiment 2: Preparation of low-molecular-weight heparin calcium nanoliposomes

[0031] Chitosan 1g is dissolved in the acetic acid solution 50mL of 2% concentration, obtains the chitosan solution of acidification, filters, gets filtrate, and in the filtrate, drips the low molecular weight heparin calcium (Changzhou Tiantian) that concentration is 20% in the filtrate under magnetic stirring General Pharmaceutical Co., Ltd., batch number H1999007) aqueous solution until a milky white precipitate appears, the resulting suspension is centrifuged, and the precipitate is freeze-dried to obtain low-molecular-weight heparin calcium nanoliposomes, and its nano-encapsulation efficiency is as high as 98% (98.06 ±1.10%).

Embodiment 3

[0032] Embodiment 3: Preparation of low molecular weight heparin calcium nanocapsules

[0033] The low-molecular-weight heparin calcium nanoliposomes obtained by the method in Example 1 were evenly divided into 20 enteric-coated capsules to obtain the low-molecular-weight heparin calcium nanocapsules, with a drug content of 50 mg / capsule.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a calcium heparin/sodium salt nano-sized oral preparation and a preparation method thereof, and a calcium heparin/sodium salt nano-sized liposome with high encapsulation rate for preparing the calcium heparin/sodium salt nano-sized oral preparation. The calcium heparin/sodium salt nano-sized liposome is obtained by the following steps: dissolving chitosan in 1-10% diluted solution of acetic acid (the usage amount of chitosan is 0.5-5g per 100ml acetic acid solution) to obtain an acidified chitosan solution, filtering, collecting filtrate, adding dropwise 1-99% aqueous solution of calcium heparin/sodium salt into the filtrate under magnetic stirring until milk white precipitate occurs, centrifugating the resulating suspension, collecting precipitates, and freezes-drying to obtain calcium heparin/sodium salt nano-sized liposome. The calcium heparin/sodium salt nano-sized oral preparation is characterized in that the preparation has good stability, high encapsulation rate (up to 98%), and high in vitro release rate (up to 25% at hour 4); the preparation is absorbed in the intestinal tract with high absorption rate and high concentration; the half life is prolonged upon the increase of oral dosage, showing zero-order kinetics; the tissue concentration is larger than blood concentration; the preparation is released sustainedly; and study in rabbits (20mg oral intake) shows that the blood concentration in vivo is kept high for over 50 hours, thereby proving sustained release.

Description

(1) Technical field [0001] The invention relates to a heparin calcium / sodium nanometer oral preparation, a preparation process and a method thereof, and a heparin calcium / sodium nanometer liposome used for preparing the heparin calcium / sodium nanometer oral preparation. (2) Background technology [0002] Heparin is a commonly used natural anticoagulant. It has been used clinically for more than 70 years. It is mainly used to treat hypertension, cardiovascular disease, stroke, thrombosis, nephrotic syndrome and other diseases. It is widely used and has more than 20 functions. mechanism. Recent studies have found that heparin can inhibit the proliferation and metastasis of malignant tumors, and increase the 5-year survival rate of patients with malignant tumors by more than 90%. However, heparin is only available as an injection, which is inconvenient to use. [0003] In the long-term clinical work, it was found that the patient's compliance with injection heparin was poor, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/00A61K9/127A61K31/727A61P7/02A61P35/00
Inventor 褚红女
Owner 褚红女
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap