Paragonimiasis detection reagent kit and method for making same

Abstract

The invention discloses a device for detecting paragonimiasis and preparation method, comprising a detector bar, a sample diluent, wherein the detection antigen is the lung fluke ESP protein represented by the amino acid sequence SEQ ID NO.2. The preparation method comprises preparing the lung fluke ESP protein and polyclonal antibody thereof, and preparing the labelled lung fluke ESP protein, the detection layer, the sample pad, the absorption pad and the detector bar and kit. The detection device prepared by the preparation method has features: high specificity, sensitivity, small cross reaction with the clonorchiasis and schistosomiasis, false positive rate lower than ELISA method, with curative effect evaluating function. The detection result becomes negative in 3-6 months after curing the paragonimiasis by effective medicine and the negative rate is higher than the ELISA method and the device for detecting paragonimiasis can accurately, quickly, simply perform diagnosis, differential diagnosis and evaluation of the curative effect with important clinical application value.

Description

technical field [0001] The invention relates to a detection kit, in particular to a paragonimiasis detection kit and a preparation method thereof. Background technique [0002] Paragonimiasis (Paragonimiasis) is a common and important zoonotic parasitic disease, mainly prevalent in Asia, Africa and Latin America, especially in Asia, prevalent in 22 provinces, municipalities and autonomous regions in my country, It is estimated that about 2 million people are infected every year, seriously endangering human health, especially the health of adolescents and children. [0003] Paragonimiasis is mainly caused by mechanical damage caused by paragonimiasis (larvae or adults) migrating and living in human tissues and organs, and immunopathological reactions caused by their metabolites. In my country, the pathogenic parasites are mainly lung fluke wescheri and lung fluke steineri, and their adults parasitize in the muscles and organs of carnivorous animals such as humans, dogs, civet...

AI Technical Summary

Problems solved by technology

[0005] In view of this, in order to solve the technical problems such as unsatisfactory curative effect assessment, cross-reaction interference, and inability to compare the test results of various laboratories in the antibody detection method of the existing paragonimiasis immunological detection method, the inventor adopted Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE), western blotting, mass spectrometry Analyzing the parasite protein at the stage, identifying the specific antigen that can be used for immune detection in the parasite excretion, and obtaining its coding gene fragment by reverse transcription polymerase chain reaction (RT-PCR) (Genbank accession number is AY083923 ), the polypeptide deduced according to the obtained sequence is composed of 165 amino acids, named as Excretory-secretory protein (Excretory-secretory protein, ESP), the protein is prepared and the specific antibody is prepared through animal immunization, and then the paragonimiasis is further obtained Detection kit

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