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Production method of reagent for detecting alcoholicity in saliva

A technology of alcohol content, applied in biological testing, material analysis through observation of the influence of chemical indicators, material inspection products, etc., can solve the problems of difficult execution of punishment basis and lower detection results, etc., to achieve the elimination of inhibitory reactions, The effect of reducing interference and accurate response results

Inactive Publication Date: 2013-04-17
SHANGHAI GAOFENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, for people with high alcohol consumption, after drinking heavily, the test results will decrease instead. Therefore, it is difficult to implement the test as a basis for punishment when implementing traffic regulations.

Method used

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  • Production method of reagent for detecting alcoholicity in saliva
  • Production method of reagent for detecting alcoholicity in saliva
  • Production method of reagent for detecting alcoholicity in saliva

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Prepare the first phase immersion liquid

[0044] Dissolve 100 iu of alcohol oxidase in 10 mL of 0.2 mol / L Tris-malonic acid buffer at pH 7.2, then add 2500 iu of horseradish peroxidase, stir well, and after fully dissolving, add acetaldehyde dehydrogenase 100iu, NAD + 20mg, 20mg gelatin (pre-dissolved).

[0045] 2. Soak it evenly with No. 3 whatman quantitative analysis test paper, take it out, absorb the excess liquid, and blow dry in an incubator at 28°C.

[0046] 3. Prepare the second phase immersion liquid

[0047] Weigh 100 mg of TMB and dissolve it with 10 mL of dimethylformamide.

[0048] The dried filter paper for leaching the first phase immersion liquid is immersed again in the second phase immersion liquid, taken out quickly, and dried in an incubator at 20°C.

[0049] 4. Paste the leached dry filter paper on a plastic card with double-sided tape, and cut into strips, each with a length of 8cm, with a 0.5×0.5cm test paper block on the top. ( figure 2 )

Embodiment 2

[0051] 1. Dissolve 200iu of alcohol oxidase in 10mL of 0.5mol / L PB buffer at pH 7.8, then add 1000iu of horseradish peroxidase, stir evenly, and after fully dissolving, add 20iu of acetaldehyde dehydrogenase, NAD + 10mg BSA (Bovine Serum Albumin) at a final concentration of 3% (w / v).

[0052] 2. Soak it evenly with No. 3 whatman quantitative analysis test paper, take it out, absorb the excess liquid, and blow dry in an incubator at 28°C.

[0053] 3. Prepare the second phase immersion liquid

[0054] Weigh 100 mg of TMB and dissolve it with 10 mL of dimethylformamide.

[0055] The dried filter paper for leaching the first phase immersion liquid is soaked again in the second phase immersion liquid, taken out quickly, and blow-dried in an incubator at 28°C. Paste the leached dry filter paper on a plastic card with double-sided tape, and cut it into strips, each with a length of 8 cm, with a 0.5×0.5 cm test paper block on the top. ( figure 2 )

Embodiment 3

[0057] 1. Prepare acetaldehyde assay reagent block soaking solution

[0058] Dissolve 120iu acetaldehyde dehydrogenase and NAD in 10 mL of 0.2mol / L Tris-malonic acid buffer at pH 7.2 + 50mg, diaphorase 60iu, nitrotetrazolium chloride 200mg, bovine serum albumin 100mg.

[0059] 2. Soak the No. 3 Whatman quantitative analysis test paper evenly, take it out, absorb the excess liquid, and blow dry in an incubator at 28°C.

[0060] 3. Paste the leached dry base paper on a plastic card with double-sided tape as the second measuring block and cut it into strips. ( image 3 )

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Abstract

The invention relates to a preparation method of a reagent for detecting the alcohol content in saliva, comprising: 1) the excessive immersion liquid is absorbed after a filter paper is soaked in the first-phase immersion liquid, the rapid warm-hot drying is carried out at the temperature of 20 to 30 DEG C, then the soaking in the second-phase immersion liquid is carried out, the re-drying is carried out at the temperature of 20 to 30 DEG C and a piece of original paper of a piece of measurement test paper is obtained; 2) the original paper of the measurement test paper is adhered on a plastic sheet, and the small measurement test strips are formed by cutting. The method can accelerate the reaction process; the reaction products are removed, so as to be conductive to the more complete reaction; when the sample to be detected contains higher concentration of ethanol, the interferences of the reactants and the reaction products can be reduced; the method is conductive to remove the inhibitory reaction in the measurement of the people with high alcohol content; the method can also be provided with two test blocks simultaneously on the same test strip, thus having more accurate judgment of the result.

Description

Technical field [0001] The invention belongs to the field of detecting alcohol in saliva, and particularly relates to a preparation method of a reagent for detecting alcohol content in saliva. Background technique [0002] The social application of alcohol testing has been extensive. In recent years, criminal cases caused by drinking and traffic accidents caused by drunk driving have been on the rise. Many countries have formulated the relationship between blood alcohol concentration and the determination of liability for traffic accidents, and established a variety of methods to detect blood alcohol content. However, in practice, taking blood samples to test the alcohol content always has health and safety risks, so it is more favored to take saliva for alcohol testing. [0003] At present, there are many test reagent strips that are easy to carry, such as the publication numbers CN1837821A and CN1904619A. They all use the following principles: [0004] [0005] [0006] ALO: Alco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/98G01N21/78
Inventor 管利丰王福进孙大尼
Owner SHANGHAI GAOFENG TECH