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Method for extracting Thalictrum aquilegifolium total alkaloids and total saponins from Thalictrum aquilegifolium

A technology for total alkaloids and extracts, which is applied in the field of preparation of Tang pine grass biological extracts, can solve the problems of low extraction purity and waste of resources, and achieve the effects of improving extraction efficiency, saving resources and being easy to regenerate.

Active Publication Date: 2008-10-08
CHONGQING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a low-cost, high-yield, suitable Preparation method of total alkaloids and total saponins of Pinus tangerata for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Weigh 1Kg of dried whole herb powder of Tangsongcao, add 10L of water and stir, control the temperature at 70°C, extract Tangsongcao powder three times, each extraction time interval is 1h, combine the extracts, filter, and the filtrate is -0.05~0.09 Under the conditions of Mpa and 50-65°C, the solvent is recovered under reduced pressure, and the crude extract of Pinus tangus is obtained by vacuum concentration. Add appropriate amount of water to the crude extract of Pinus tangerata and stir it into a suspension, so that the specific gravity of the suspension reaches 1.20. The HPD700 model macroporous adsorption resin is used to absorb the suspension, enrich and adsorb to saturation, and use 2 times the amount of resin water (V / V) Wash away the impurities, then use 5 times the amount of resin (V / V) for analysis with methanol with a concentration of 50%, collect the analysis solution, control the temperature at 50-65°C to recover the solvent under reduced pressure, and d...

Embodiment 2

[0018] Weigh 1Kg of dried whole herb powder of Tangsongcao, add 10L 50% ethanol and stir, control the temperature at 85°C, carry out reflux extraction of Tangsongcao powder for 3 times, each extraction time interval is 1h, combine the extracts, filter, and the filtrate is at -0.05 Under the conditions of ~0.09Mpa and 50~65°C, the solvent is recovered under reduced pressure, and the crude extract of Pinus tangerata is obtained by vacuum concentration. Add appropriate amount of water to the crude extract of Pinus tangerata and stir it into a suspension, so that the specific gravity of the suspension reaches 1.11. The suspension is adsorbed by the AB-8 model macroporous adsorption resin, enriched and adsorbed to saturation, and the water with 2 times the amount of resin ( V / V) to wash away impurities, then use 4 times the amount of resin (V / V) to analyze with ethanol with a concentration of 70%, collect the analysis solution, control the temperature at 50-65°C to recover the solve...

Embodiment 3

[0020]Weigh 1Kg of dried whole herb powder of Tangsongcao, add 10L of 70% ethanol to stir, control the temperature at 88°C, carry out reflux extraction of Tangsongcao powder for 3 times, each extraction time interval is 1h, combine the extracts, filter, and the filtrate is at -0.05 Under the conditions of ~0.09Mpa and 50~65°C, the solvent is recovered under reduced pressure, and the crude extract of Pinus tangerata is obtained by vacuum concentration. Stir the crude extract of Pinus tangerata with an appropriate amount of water to form a suspension, so that the specific gravity of the suspension reaches 1.09, and use XDA-1, AB-8 (1:1) mixed macroporous adsorption resin to adsorb the suspension, enriching and adsorbing to Saturation, wash away impurities with 2 times the amount of resin (V / V), then use 5 times the amount of resin (V / V) to analyze with ethanol with a concentration of 50%, collect the analysis solution, and control the temperature at 50-65 ° C to reduce The solve...

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PUM

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Abstract

The invention relates to a method for extracting Thalictrum squarrosum total alkaloids and total saponins from grass of meadow rue, pertaining to biological and pharmaceutical technical field. The invention discloses the method for simultaneously preparing extracts of total alkaloids and total saponins from grass of meadow rue. The invention uses an absorption resin for absorbing the total alkaloids and the total saponins from grass of meadow rue, an organic solvent or the water solution thereof is utilized to desorb the absorption resin; then an ion exchange resin is used for selectively absorbing alkaloids from grass of meadow rue extract, thus obtaining the saponins from eluate. The method of the invention enhances the extracting efficiency of effective group of components from grass of meadow rue, avoids the loss of effective components, and saves the sources; the content of the extract of total alkaloids purified by using the method is higher than 50 percent, and the content of the extract of total saponins is higher than 30 percent.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Specifically, it relates to a preparation method of pine grass biological extract. Background technique [0002] Thalictrum L. plants of Ranunculaceae have anticancer, antibacterial and anti-inflammatory, antiviral, antiarrhythmic, antimalarial, immune regulation, antihypertensive, heat-clearing and detoxifying, analgesic, diuretic and other effects. The plants of the genus Echinacea are widely used in national folk medicine. There are 67 species of plants in this genus that are used in 15 nationalities in my country. They are an important part of Tibetan medicine, Mongolian medicine and many other nationalities. Due to the geographical and climatic environment, more than half of the plants in this genus are endemic in my country, and the resources are abundant. Tang pine grass contains alkaloids and triterpenoid saponins, which are the main medicinal active ingredients in tang pine grass. ...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P9/12A61P31/04A61P35/00
Inventor 潘正高运玲蔡应繁
Owner CHONGQING UNIV OF POSTS & TELECOMM
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