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Anti-oxidation peptide from bee milk

A technology for antioxidant peptides and royal jelly, which is applied in the fields of peptides, cosmetic preparations, chemical instruments and methods, etc., can solve the problems of backward research on physiological effects, lack of effective utilization of protein, and less effect than vitamin E, etc.

Inactive Publication Date: 2008-10-15
YAMADA BEE COMPANY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, although royal jelly generally contains more than 10% protein, research on the molecular structure and physiological functions of the protein has been lagging behind.
In addition, when producing cosmetics and health potions (drinker) containing royal jelly, alcohol extraction is carried out, and the protein is separated as a precipitate, but the effective utilization of this protein has not been realized at this stage
[0006] The protease-treated product of royal jelly protein is known to have calcium absorption-promoting action (Keiko Nakasa, Food and Kaizhen, 34, 5 (1999)), blood sugar-lowering action (Shuichiro Ueda, Food Style, 21, 6, 5 (2002)), and there is also a report on its antioxidant effect ((company) Japan National Royal Jelly Fair Trade Council (National Royal Jelly Fair Citation Council) "Royal Jelly (Royal Jelly)に关する Research" Heisei 13 Annual Research Report), but its effect is not as good as that of vitamin E

Method used

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  • Anti-oxidation peptide from bee milk
  • Anti-oxidation peptide from bee milk
  • Anti-oxidation peptide from bee milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Modulation of antioxidant peptide fraction

[0039] After freeze-drying the alcohol-denatured royal jelly protein, it was dissolved in 5 times the amount of distilled water. Low-molecular sugars and impurities are removed by dialysis to obtain alcohol-denatured royal jelly protein with higher purity. The protein solution was adjusted to pH 2.8 with 1N HCl and initially treated with 1 / 500 weight ratio of pepsin. The decomposed product was sequentially treated with trypsin, chymotrypsin (manufactured by Sigma), and pig-derived small intestine enzyme. Pepsin, trypsin, and chymotrypsin were added and incubated at 37°C for 24 hours, and intestinal enzymes were added and incubated at 37°C for 1 hour. When the reaction is stopped, if it is pepsin, adjust the pH to 8.0 with 1N NaOH to inactivate the enzyme, if it is trypsin, chymotrypsin, or intestinal enzyme, add 4 times the amount of 99.7% ethanol at 3,000 × g Centrifuge for 20 minutes to remove unreacted protein and e...

preparation example 1

[0054] Preparation Example 1: lotion

[0055] Combine the following ingredients and make lotion according to the usual method.

[0056] Ethanol 5% by weight

[0057] Glycerin 4% by weight

[0058] BG (1,3-butanediol) 4% by weight

[0059] 0.5% by weight of the royal jelly protein enzymatic hydrolyzate obtained in Example 1

[0060] Citric acid amount

[0061] Sodium citrate amount

[0062] purified water residual

preparation example 2

[0063] Preparation Example 2: Health Potion

[0064] Cooperate with the following ingredients, and prepare health care liquid according to the usual method.

[0065] Water 88% by weight

[0066] Glucose 10% by weight

[0067] Sodium ascorbate 4% by weight

[0068] Royal jelly protein enzymatic hydrolyzate obtained in embodiment 1 1% by weight

[0069] Citric acid amount

[0070] EDTA2Na 0.5% by weight

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Abstract

The antioxidative peptide has an amino acid sequence described in either one of the formulas (1) to (3). (1) Tyr-Tyr-Ser-Pro-Leu; (2) Asn-Tyr-Pro-Phe-Asp-Val-Asp-Gln; and (3) Ser-Leu-Pro-Ile-Leu-His-Glu-Trp.

Description

[0001] This application is a divisional application of a Chinese patent application with an application date of February 16, 2005 and an application number of 200510009423.0. technical field [0002] The present invention relates to a peptide having an antioxidant effect, and specifically relates to an antioxidant peptide obtained by hydrolyzing a protein contained in royal jelly. Background technique [0003] Now, royal jelly is widely used in health food, pharmaceuticals, cosmetics and other fields. Reports show that it has various pharmacological and nutritional physiological effects such as dilating blood vessels, lowering blood pressure, and antibacterial. [0004] Royal jelly is a milky substance synthesized by the salivary glands of honeybees and secreted by the mandibular glands located in the head. It is a substance that worker bees feed to young bees in the queen bee larva nest in order to cultivate the queen bee. Royal jelly is rich in protein, vitamins, and miner...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C09K15/16C12P21/06A23L1/305A61K8/64A23L1/30C09K15/06
Inventor 西村敏英田边创一杂贺爱
Owner YAMADA BEE COMPANY INC