Unlock instant, AI-driven research and patent intelligence for your innovation.

Process for efficiently cracking chlamydia cell wall

A chlamydia and cell wall technology, applied in the field of microbiology, can solve the problems of loss of biological activity, nuclear protein denaturation, chlamydia cell wall rupture, etc., and achieve the effect of improving sensitivity and accuracy

Inactive Publication Date: 2010-05-19
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlamydia has a cell wall composed of mucinous peptides. The conventional method of cracking the cell wall of Chlamydia is: freeze the Chlamydia sample at -20 to -40°C, take it out and thaw it at room temperature, and then freeze it again. The cell wall is broken to release biologically active substances in the medium; or it is destroyed by carbolic acid, papain or heated at 60°C. Such methods will cause denaturation of nuclear proteins and lose their biological activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for efficiently cracking chlamydia cell wall
  • Process for efficiently cracking chlamydia cell wall
  • Process for efficiently cracking chlamydia cell wall

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Adopt the method of the present invention to crack chlamydia samples: collect 63 feces samples (parts / individual) of pigs raised by 24 farmers in a certain county of Hubei Province, after the samples are aseptically treated, inoculate the yolk sac cavity of 6-8d-old chicken embryos under aseptic conditions , cultured at 37°C for 7 days, collected the yolk sac membrane of dead chicken embryos at 3 to 6 days, and made yolk sac membrane homogenate with a tissue homogenizer. Design the specific primers of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia psittaci respectively, carry out polymerase chain reaction (PCR) detection experiment, the result is as follows:

[0011]

Embodiment 2

[0019] Adopt the method of the present invention to crack Chlamydia samples: collect 110 feces samples (parts / individual) of ornamental parrot birds raised by 3 farmers in a certain county of Hubei Province, after the samples are aseptically treated, inoculate the yolk sac of 6-8d-old chicken embryos under aseptic conditions Cavity, cultured at 37°C for 7 days, collected the yolk sac membrane of 3-6 day dead chicken embryos, and made yolk sac membrane homogenate with tissue homogenizer. Design the specific primers of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia psittaci respectively, carry out polymerase chain reaction (PCR) detection experiment, the result is as follows:

[0020]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a method for cracking cell wall of Chlamydia with high efficiency. The method is characterized by placing a sample of Chlamydia at a temperature of 80 DEG C below zero for 10 to12 hours; putting the sample of Chlamydia into a vacuum cup which is pre-cooled at a temperature of about 50 DEG C below zero after being taken out; and maintaining subpressure of 5.5 multiplied by 0.1mbar for 2 to 3 hours. The method of the invention sufficiently releases substances in a cell and maintains the complete biological activity of the cell, thereby improving sensitivity and accuracy for a molecular biological testing method to test the infection of the Chlamydia.

Description

technical field [0001] The invention relates to the field of microbiology, genus Chlamydia (Chlamydia), and the lysing technology of chlamydia cell walls of different chlamydia species, and in particular provides a method for efficiently lysing chlamydia cell walls, fully releasing intracellular substances, and maintaining its complete biological activity. The sensitivity and accuracy of the molecular biological detection method for detecting chlamydia infection have been improved. technical background [0002] Chlamydia is susceptible to humans, mammals and poultry, causing pneumonia, conjunctivitis, rhinitis and diarrhea, etc., and is characterized by abortion and pneumonia in livestock. It is a very important pathogen of natural foci of zoonotic diseases. It endangers the health of people, livestock and poultry, and has a great impact on my country's public health. Chlamydia belongs to the genus Chlamydia (Chlamydia) in biological classification, and is a group of microor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06
Inventor 李天宪吴勇敢唐霜李永东陈绳亮范兆军张忠
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI