Mutant strain streptomyces albus TUST2 and process for producing epsilon-polylysine and salts thereof by using the mutant strain

A technology of Streptomyces albicans and TUST2, which is applied in the field of fermentation engineering to achieve the effect of high generation capacity and improved yield

Inactive Publication Date: 2011-08-17
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

However, it is still unsatisfactory in terms of ε-PL yield and glucose conversion efficiency

Method used

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  • Mutant strain streptomyces albus TUST2 and process for producing epsilon-polylysine and salts thereof by using the mutant strain
  • Mutant strain streptomyces albus TUST2 and process for producing epsilon-polylysine and salts thereof by using the mutant strain
  • Mutant strain streptomyces albus TUST2 and process for producing epsilon-polylysine and salts thereof by using the mutant strain

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Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with specific examples, and the following examples are only descriptive, not limiting, and cannot limit the protection scope of the present invention with this.

[0028] First things first:

[0029] 1. Streptomyces albicans TUST2, a mutagenic strain that produces a large amount of ε-polylysine, has been preserved by the General Microbiology Center of China Committee for Culture Collection of Microorganisms. The preservation number is CGMCC No.1986, and the preservation date is March 23, 2007.

[0030] 2. The mutagenized strain TUST2 of the present invention is obtained by mutagenizing the enrichment medium added with ε-polylysine from the soil of Hainan Island to select the high-yielding ε-polylysine production strain TUST1 as the starting strain , which is resistant to S-(2-aminoethyl)-L-cysteine ​​at a concentration of 10 mg / ml or higher.

[0031] 3. The AEC used in the present invention i...

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Abstract

The invention relates to a mutagenized strain of streptomyces albus TUST2, and a method for using the same to produce epsilon-polylysine and a salt of the epsilon-epsilon. The strain is a high-yield mutagenized strain of epsilon-polylysine selected and bred by means of ultraviolet mutagenesis, ultraviolet-chemical mutagenesis, N ion injection mutagenesis, etc from a strain of streptomyces albus TUST2 which is capable of producing epsilon-polylysine and is screened from the earth in Hainan province of China; the mutagenized strain is resistant to 10mg / ml or above S-(2 Aminoethyl )-L-Cysteine and can generate 10 to 30 g / L acid under optimal conditions; zymotic fluid is removed of the streptomyces albus by configuration and is filtered by diatomaceous earth to obtain clear filtrate and to weak acid type ion exchange resin to obtain coarse epsilon-polylysine products which is subjected to ultrafiltration and purification to get epsilon-polylysine and the salt of the epsilon-epsilon. The distribution of molecular weight of epsilon-polylysine is between 4000 and 6500Da.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a mutagenic strain Streptomyces albicans TUST2 that produces a large amount of ε-polylysine and a method for producing ε-polylysine and its salt by using the mutated strain TUST2 by fermentation . Background technique [0002] ε-poly-L-lysine (ε-poly-L-lysine, ε-PL for short) is an L-lysine homopolymer synthesized by microorganisms, which is formed by linking ε-amino and α-carboxyl groups through peptide bonds. ε-polylysine has anti-Gram-positive bacteria, Gram-negative bacteria, fungi and viruses, and is an excellent biological preservative, widely used in cosmetics, gene carriers, drug coatings, electronic materials and environmentally friendly materials. [0003] The method for fermentatively producing ε-PL in the prior art is known to have a method (approved Japanese patent publication 59-20359), comprising cultivating Streptomyces parvus lysin...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12P13/08C12N13/00C12R1/47C12N1/20
Inventor 贾士儒谭之磊袁国栋曹伟锋赵颖欧竑宇李力贺新义邓子新
Owner TIANJIN UNIV OF SCI & TECH
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