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Three classifications whole blood quality control substance simulant and method for making same

A simulant and three-classification technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of deviation of hematological characteristics, difficulty in preparing human platelets, high cost, etc., and achieve the effect of long shelf life and good quality control effect

Active Publication Date: 2008-10-15
SICHUAN MACCURA BIOTECH CO LTD
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Problems solved by technology

Due to different regions, the hematological characteristics of the same species may deviate. Local materials will save huge costs, and the existing quality control materials also have some defects. It is difficult to prepare pig platelets to simulate human platelets, and they are easy to aggregate; sheep red blood cells are fixed and then added It is prone to false white blood cell interference; human red blood cells suffer a certain blow during processing, and the validity period is not long

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  • Three classifications whole blood quality control substance simulant and method for making same

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Embodiment Construction

[0017] like figure 1 As shown, the preparation method of the three-classification whole blood quality control substance simulant of the present invention comprises the preparation of red blood cell simulant, the preparation of white blood cell and platelet simulant:

[0018] (1) Preparation of erythrocyte simulants: a. Collect waste blood from livestock or plasma stations and treat it with an anticoagulant. The anticoagulant component is: EDTA-Na 2 50~150g / L, sodium citrate 20~40g / L, anticoagulant is sterilized by autoclaving after the above-mentioned composition preparation, volume ratio is anticoagulant by anticoagulant: blood=1: 10~20 carries out anticoagulant treatment; b, Use washing liquid to wash the discarded blood of domestic animals or plasma stations treated in step a, remove plasma and buffy coat by centrifugation to prepare red blood cells, and the purity of the prepared red blood cells is 99-100%; c, use semi-fixed liquid to prepare red blood cells in step b Se...

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Abstract

The invention relates to a method for preparing three-classification whole blood control substance simulacrum and a blood type detection reagent box made by the method. The method comprises the preparation of red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum. The concentration of the prepared red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum is adjusted to ensure that cell particle is suspended in cell suspension and is used as monomial substance control substance, thereby forming whole blood control substance. The invention aims to solve the source of various cells and provides a method for preparing three-classification whole blood control substance. The invention adopts fish blood to simulate human white blood cell and blood platelet, and waste red blood cell of a livestock red blood cell plasma-mixing station to simulate human red blood cell, thereby simulating the practical histogram distribution of human blood sample; moreover, the cell suspension adopting the formula of the invention has longer storage life; in addition, the method can carry out simulation operation when adopting the red blood cells of silver carp, grass carp, common carp and crocodile, etc. during experiment, and can obtain excellent quality-control effect through fixative treatment.

Description

technical field [0001] The invention relates to a three-classification whole blood quality control substance simulant and a preparation method thereof. Background technique [0002] Whole blood quality control is one of the important reagents used in the field of clinical testing for intra-experimental and inter-laboratory quality evaluation. Foreign quality control materials have stable performance. At present, domestic related products mainly rely on imports. Through the search of relevant domestic and foreign literature and patents, Foreign countries mainly use bird nucleated red blood cells, latex, pollen, microbial particles, ungulate red blood cells or naked nuclei and other simulated white blood cells and platelets, and use human red blood cells to simulate red blood cells as quality controls. Due to the special policy on blood source in China, the problem of blood source needs to be solved urgently. Due to different regions, the hematological characteristics of the ...

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Application Information

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IPC IPC(8): G01N33/96G01N33/80
Inventor 李恒王保宁杨慧杨卫平王俊
Owner SICHUAN MACCURA BIOTECH CO LTD
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