Activation method for enzyme of glutamic acid decarboxylase (GAD) in rice polishings

Abstract

The invention discloses a method for activating glutamic acid deearboxylase in rice bran, in particular to a method for preparing rice bran rich in gamma-aminobutyric acid by activating glutamic acid deearboxylase in rice bran by enzyme biotechnology, which belongs to the field of food biotechnology. The method comprises the following steps: adding glutamic acid (Glu), sodium glutamate (Glu-Na), calcium chloride, pyridoxal-5-phosphate and VB6, adjusting pH value by using phosphate buffer or ascorbic acid or lactic acid, activating activity of GAD at a certain temperature, and reacting to obtain a reactive liquid rich in gamma-aminobutyric acid (GABA). The method can be used for preparing high-concentration GABA rice bran and for producing GABA from rice bran. Accordingly, rice bran has certain economic and social significance and can be further developed and utilized as a material for health food with abundant nutrient and excellent physiological function.

Description

technical field [0001] The invention relates to an enzyme activation method of glutamic acid decarboxylase in rice bran, which specifically relates to a method for preparing rice bran rich in γ-aminobutyric acid by using enzyme biotechnology to activate glutamic acid decarboxylase in rice bran, and belongs to the field of food biotechnology. Background technique [0002] In the process of rice processing, brown rice is first obtained by removing the husk and husk of rice, and secondly, white rice is obtained by removing the brown cortex outside the brown rice. The main by-product rice bran includes aleurone layer, nacelle layer, pericarp layer and the interlayer Several sublayers, but the bran in actual production is also mixed with rice germ and broken rice. Rice is the largest grain variety in my country, with an annual production of about 185 million tons, accounting for 42% of the country's total grain output. Rice bran is a by-product of rice processing. Rice bran acco...

AI Technical Summary

Problems solved by technology

Since microbial GAD is an intracellular enzyme, Escherichia coli cells are generally used as the enzyme source for the production of GABA, but this GABA product cannot be used in food