Alkylamine silica gel capillary monolithic column and preparation method and use thereof

A capillary monolithic column, alkylamine technology, applied in separation methods, chemical instruments and methods, other chemical processes, etc., can solve the problem of few silica-based monolithic columns, and achieve silica-based matrix network permeability and high mechanical strength, Increased hydrophilicity and solvent resistance

Inactive Publication Date: 2008-11-19
GUANGXI NORMAL UNIV
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ding et al. (GuoshengDing, Zhenliang Da, Ruijuan Yuan, James J.Bao, Electrophoresis, 2006, 27, 3363-3372) prepared a reverse-phase-weak anion exchange mixed-mode silica-based monolithic column, but it is applied to polar and alkaline There are few reports on silica-based monolithic columns for active compounds (Sebastiaan Eeltink Wim Th.Kok Electrophoresis 2006, 27, 84-96)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alkylamine silica gel capillary monolithic column and preparation method and use thereof
  • Alkylamine silica gel capillary monolithic column and preparation method and use thereof
  • Alkylamine silica gel capillary monolithic column and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of silica gel monolithic column: Take a quartz capillary with an inner diameter of 75 μm and an outer diameter of 365 μm, soak it with 1 mol / L NaOH solution overnight, rinse with secondary water, hydrochloric acid, secondary water, acetone, and anhydrous ether for 1 hour, Then pass N 2 Dry at 180°C for 3 hours, and seal both ends for later use. 0.22g of polyethylene glycol (Mr=10000), 0.225g of urea were dissolved in 2.5mL of 0.01mol / L acetic acid solution, wherein 1mL of tetramethoxysilane and methyltrimethoxysilane (9 / 1, v / v), stir vigorously in an ice bath until the solution becomes a transparent and uniform sol, inject it into the pretreated capillary, seal both ends with silicone rubber, put it in a constant temperature water bath at 40°C for 25h, take it out, and store it at 120°C Under heat treatment for 3h to obtain proper specific surface and pore structure. Cut off the seal, rinse out unreacted substances with water, rinse with water and abs...

Embodiment 2

[0047] Using 2.5 g of n-decylamine instead of n-tetradecylamine, the procedure in Example 1 was followed to obtain a n-decylamine capillary monolithic column.

Embodiment 3

[0049] Using the alkylamine silica gel capillary monolithic column prepared in Example 1, the cut-off total length is 30cm, and the effective length is 20cm. , v / v) buffer solution is the mobile phase, under the conditions of applied voltage -15kV and detection wavelength 214nm, the homologues of benzene are separated, and the corresponding peaks are 1. benzene, 2. toluene, 3. ethylbenzene, 4. propylene Benzene, 5. Butylbenzene, 6. Pentylbenzene, 7. Hexylbenzene. Its spectrum is as Figure 4 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a novel reversed phase electrochromatography silica-based monolithic column, that is, an alkylamine silica capillary monolithic column which can realize the separation and the analysis of most of organic compounds containing neutral, acidic and alkaline compounds on the premise of meeting the simple chromatographic conditions. The alkylamine silica capillary monolithic column can effectively improve the symmetry of chromatographic peaks in particular to the alkaline organic compounds. The alkylamine silica capillary monolithic column of the invention comprises a capillary and a fixed phase, in the structural formula of the fixed phase: Si represents the silica capillary monolithic column, R represents normal chain alkyl and R1 represents hydrogen or the normal chain alkyl. The invention further provides a preparation method of the alkylamine silica capillary monolithic column, and the specific synthesis steps are as follows: the silica capillary monolithic column is firstly prepared, then the silica capillary monolithic column containing epoxy is prepared and the alkylamine silica capillary monolithic column is finally obtained by connecting alkylamine. The invention further provides the application of the alkylamine silica capillary monolithic column in neutral, acidic and alkaline organic compounds.

Description

technical field [0001] The invention relates to a capillary electrochromatographic column, especially an alkylamine silica gel capillary monolithic column, a preparation method and application thereof. Background technique [0002] Capillary electrochromatography (CEC) is a new separation and analysis method that combines the two technical advantages of high performance liquid chromatography and high performance capillary electrophoresis. It has the characteristics of high efficiency, high selectivity, high resolution and rapid separation. CEC has opened up a new approach in the field of high-efficiency microanalysis and has become a new direction for the development of contemporary chromatography (Luo Guoan, Wang Yiming, Chen Lingxin, Liang Qionglin, Capillary Electrochromatography and Its Application in Life Sciences. Beijing, 2005, Science Press). [0003] The commonly used electrochromatographic columns are particle-packed columns. The filling of this type of column and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/22B01J20/291B01J20/283G01N30/60
Inventor 叶芳贵赵书林王顺
Owner GUANGXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap