Method for separating benzoxazole oxazinone glycoside compounds from acanthus

Abstract

The invention relates to a method for separating a benzoxazine ketone compound (R is H or OH) as shown in a formula (1) from Acanthus ilicifolius L. The method is characterized in that: an upper phase of a solvent system consisting of three compositions a, b and c according to a volume ratio of 2-5 to 3-6 to 5-10; a lower phase of the solvent system is used as a mobile phase, wherein, the composition a is ethyl acetate, propyl acetate or isopropyl acetate; the composition b is normal butanol, isobutyl alcohol or pentanol; the composition c is water or a hartshorn; a normal butanol extractant of the Acanthus ilicifolius L is dissolved in the solvent system to prepare a sample solution; flow fractions are detected by an ultraviolet spectrograph through a high-speed countercurrent chromatography and collected according to a chromatographic peak, and the compound with the formula (1) is obtained, wherein R represents H or OH. The method can save mass organic solvents; a separation processonly needs 3 to 6 hours; the produced compound has a purity of over 80 percent; and the product can be directly used for standard chromatograpical samples, pharmacological experiments or business applications.

Description

technical field The present invention relates to a method for isolating benzoxazinone compounds from rat bougainvillea (Acanthus ilicifolius L.), in particular to a method for separating the following formula ( 1) Represented (2R)-2-O-β-D-glucosyl-2H-1,4-benzoxazin-3(4H)-one and (2R)-2-O-β-D- Glucosyl-4-hydroxy-2H-1,4-benzoxazin-3(4H)-one. Background technique Bougainvillea is a perennial herb, and the whole plant can be used as medicine to treat lymphadenitis, hepatitis, asthma, etc. Pharmacological studies have shown that the extract of Porcini musculus has antiviral, anticancer, antioxidative, antimutagenic, hepatoprotective and anti-inflammatory activities. At present, 6 kinds of benzoxazinone compounds have been extracted from rat bougainvillea. These compounds mainly play the role of defense against diseases and insect pests, competition with other plants through allelopathy, and growth hormone in plants. After research, these secondary metabolites can be used as n...

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Problems solved by technology

However, there is no relevant research on the application of high-speed countercurrent chromatography technology to the separation of benzoxazinone compounds in rat bougainvillea.

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