Inorganic phosphorus diagnosis/ determination reagent kit and method for determining inorganic phosphorus concentration

An inorganic phosphorus and kit technology, which is applied in the field of inorganic phosphorus diagnosis/determination kits and the determination of inorganic phosphorus concentration, can solve the problem of high results

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] The inorganic phosphorus diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0106] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0107] Stabilizer 500mmol / L

[0108] Reduced coenzyme 0.25mmol / L

[0109] Glutamine 10mmol / L

[0110] Adenosine diphosphate 20mmol / L

[0111] Pyruvate 20mmol / L

[0112] Glutamine Synthetase 10000U / L

[0113] Alanine dehydrogenase 12000U / L

[0114] Glycine oxidase 12000U / L

[0115] Peroxidase 30000U / L

[0116] 4-amino antiarsenic 2mmol / L

[0117] Carbolic acid 10mmol / L

[0118] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0119] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured inorganic phosphorus sample and reagent is...

Embodiment 2

[0122] The inorganic phosphorus diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0123] Reagent 1

[0124] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0125] Stabilizer 50mmol / L

[0126] Reduced coenzyme 0.25mmol / L

[0127] Glutamine 10mmol / L

[0128] Adenosine diphosphate 20mmol / L

[0129] Pyruvate 20mmol / L

[0130] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0131] Reagent 2

[0132] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0133] Stabilizer 500mmol / L

[0134] Glutamine Synthetase 10000U / L

[0135] Alanine dehydrogenase 12000U / L

[0136] Glycine oxidase 12000U / L

[0137] Peroxidase 30000U / L

[0138] 4-amino antiarsenic 0.6mmol / L

[0139] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0140] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10...

Embodiment 3

[0143] The inorganic phosphorus diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0144] Reagent 1

[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0146] Stabilizer 50mmol / L

[0147] Reduced coenzyme 0.25mmol / L

[0148] Glutamine 10mmol / L

[0149] Adenosine diphosphate 20mmol / L

[0150] Pyruvate 20mmol / L

[0151] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0152] Reagent 2

[0153] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0154] Stabilizer 500mmol / L

[0155] Alanine dehydrogenase 12000U / L

[0156] Glycine oxidase 12000U / L

[0157] Peroxidase 30000U / L

[0158] Dimethylaniline 2mmol / L

[0159] Reagent 3

[0160] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0161] Stabilizer 500mmol / L

[0162] Glutamine Synthetase 10000U / L

[0163] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, ...

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Abstract

The invention relates to a kit for diagnosing/mensurating inorganic phosphorus by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the inorganic phosphorus, and belongs to the technology field of medical/food/environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, glutamine, adenosine diphosphate, pyruvic acid, glutamine synthetase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into the colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by a visible light analyzer and further reflecting the concentration of the inorganic phosphorus directly.

Description

technical field [0001] The invention relates to an inorganic phosphorus diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of inorganic phosphorus, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. When the blood calcium increases in normal people, the blood phosphorus decreases, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma remains within a certain range; the product of calcium and phosphorus concentrations per 100 ml of plasma is about 35--40. When the product of the two is greater than 40, calcium and phosphorus are deposited in the bone t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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