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Method for improving chondroitin sulfate quality by protein isoelectric point
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A technology of chondroitin sulfate and isoelectric point, which is applied in fermentation and other directions, can solve the problems of low purity and low product yield, and achieve the effect of improving the efficiency of enzymatic hydrolysis
Inactive Publication Date: 2009-01-21
NANJING UNIV OF TECH +1
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Abstract
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Problems solved by technology
[0004] The purpose of the present invention is aimed at the shortcomings of low product yield and low purity in the existing dilute alkali dilute salt-enzyme extraction process, adopts relatively concentrated lye (2%) to effectively release chondroitin sulfate, and simultaneously degrade part of the protein and Denature precipitation, improve purity
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Examples
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Effect test
Embodiment 1
[0019] Use 3‰ NaHCO for beef nose bone 3 After the solution is washed, it is steamed for 9 hours; after cooling to below 30°C, NaOH and NaCl are added for extraction twice; the amount of NaOH is 2% of the cartilagemass, and the amount of NaCl is 8% of the cartilagemass.
[0020] Add the crushed and homogeneous porcine pancreas and 2709 protease to the extract, and treat it at 51°C and pH 8.8-8.9 for 7 hours. The amount of porcine pancreas added is 8% of the cartilage mass, and the amount of 2709 enzyme added It is 0.6% of cartilage quality. The enzyme-treated extract was treated for 1 hour at a temperature of 67°C and a pH of 4.4 to 4.6, and then adjusted to pH 5.5 to 6.0 to clear separation. After filtering with a 747 filter cloth, the filtrate was concentrated to 1 / 3 of the original volume.
[0021] Add alcohol to the above concentrated solution to a volume content of 64%, process 4 hours for a precipitation, filter, add water to dissolve the filter residue at 63 ℃, first stan...
Embodiment 2
[0023] Use 3‰ NaHCO for beef nose bone 3 After the solution is washed, it is steamed for 9 hours; after cooling to below 30°C, NaOH and NaCl are added for extraction twice; the amount of NaOH is 2% of the cartilage mass, and the amount of NaCl is 8% of the cartilage mass.
[0024] The homogenized porcine pancreas and 2709 protease were added to the extract, and treated at 50°C and pH 8.8-8.9 for 8 hours. The added amount of porcine pancreas was 6% of the cartilage mass, and the added amount of 2709 enzyme was 1% of cartilage quality. .
[0025] The enzyme-treated extract was treated for 1 hour at a temperature of 66° C. and a pH of 4.4 to 4.6, and then adjusted to pH 5.5 to 6.0 to clear separation, then filtered, and the filtrate was concentrated.
[0026] Add alcohol to the above concentrated solution to a volume content of 65%, process for 4 hours to perform a precipitation, filter, and dissolve the filter residue with water at 64 ℃, first stand for 1 hour at pH 5.0 to 5.5, and ...
Embodiment 3
[0028] Except for replacing the bovine nasal bone with a bovine throat tube, everything else is the same as in Example 1. The final white powder yield is 15% and the purity is 97.4%.
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Abstract
The invention discloses a process for preparing chondroitinsulfate by a double enzyme method. The process comprises the following steps: NaOH and NaCl are added after cleaning and steam boiling so as to carry out extraction; then, pancreatin is added in extraction solution so as to carry out enzymolysis treatment; the extraction solution after the enzyme treatment is deproteinzed and then is concentrated; and finally, impurityprotein is removed through primary sedimentation and pH value adjustment and then secondary sedimentation is carried out so as to obtain a crude product of chondroitinsulfate. Through accurate pH value adjustment after primary sedimentation, the process can further remove the impurityprotein which is mixed in the chondroitinsulfate and hard to remove; moreover, by means of the mutual coordination of other steps, the process ensures that the purity of the finally obtained chondroitin sulfate is more than 97 percent.
Description
Technical field [0001] The invention belongs to the field of biochemical pharmacy, and specifically relates to a preparation method of chondroitin sulfate. Background technique [0002] Chondroitin sulfate is a type of mucopolysaccharide widely found in human and animal cartilage tissue. As an important part of connective tissue, it has a variety of pharmacological and physiological functions. In my country, chondroitin sulfate is used to treat neuralgia, neuropathic migraine, arthritis, shoulder joint pain and pain after abdominal cavitysurgery, etc., to prevent and treat hearing impairment caused by streptomycin and hearing difficulties caused by various noises, tinnitus And so on, the effect is remarkable. In western developed countries, chondroitin sulfate has been used as a health care product for the prevention and treatment of coronary heart disease, angina pectoris, myocardial infarction, coronary insufficiency, myocardial ischemia and other diseases. It has no obvious s...
Claims
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Application Information
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